Detailed information of TA system TA044102

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 46135..46670
• Replicon: plasmid pEC291_1
• Accession: NZ_AP027444
• Organism: Escherichia coli strain EC291 isolate EC291

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: QUE55_RS01275
• Protein ID: WP_001270421.1
• Coordinates: 46135..46422 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: L4J0V6
• Locus tag: QUE55_RS01280
• Protein ID: WP_001132895.1
• Coordinates: 46419..46670 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QUE55_RS01240 (41443)	41443..41595	+	153	WP_227443290.1	hypothetical protein	-
QUE55_RS01245 (41646)	41646..42342	+	697	WP_286347927.1	IS1 family transposase	-
QUE55_RS01250 (42427)	42427..42721	+	295	Protein_46	type II toxin-antitoxin system RelE/ParE family toxin	-
QUE55_RS01255 (42766)	42766..42961	+	196	Protein_47	serine/threonine protein kinase	-
QUE55_RS01260 (43082)	43082..43822	+	741	WP_001066941.1	tyrosine-type recombinase/integrase	-
QUE55_RS01265 (44101)	44101..45078	-	978	WP_000361612.1	RepB family plasmid replication initiator protein	-
QUE55_RS01270 (45722)	45722..46133	-	412	Protein_50	DDE-type integrase/transposase/recombinase	-
QUE55_RS01275 (46135)	46135..46422	-	288	WP_001270421.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
QUE55_RS01280 (46419)	46419..46670	-	252	WP_001132895.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
QUE55_RS01285 (46844)	46844..47056	-	213	WP_000402944.1	hypothetical protein	-
QUE55_RS01290 (47431)	47431..48636	+	1206	WP_000817642.1	AAA family ATPase	-
QUE55_RS01295 (48633)	48633..49595	+	963	WP_032200967.1	ParB family protein	-
QUE55_RS01300 (49736)	49736..49909	-	174	Protein_56	DUF4113 domain-containing protein	-
QUE55_RS01305 (49983)	49983..50408	+	426	WP_063090491.1	antirestriction protein	-
QUE55_RS01310 (50408)	50408..50861	+	454	Protein_58	hypothetical protein	-
QUE55_RS01315 (50858)	50858..51049	+	192	WP_032200969.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	dfrA12 / aadA2 / cmlA1 / mcr-5.1 / blaTEM-1B	-	1..98615	98615

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-83)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11244.27 Da
• Isoelectric Point: 10.4703

>T44102 WP_001270421.1 NZ_AP027444:c46422-46135 [Escherichia coli]
MRYQVKFREDALKEWQKLDKTIQQQFAKKLKKCCDNPHIPSAKLRGIKDCYKIKLRASGFRLVYQVIDEQLIIAVVAVGK
RERSDVYNLASERMR

Nucleotide

• Length: 288 bp

>T44102 NZ_AP027444:c46422-46135 [Escherichia coli]
ATGAGGTATCAGGTAAAATTCAGGGAAGATGCGCTGAAAGAGTGGCAAAAACTGGACAAGACTATTCAGCAACAGTTTGC
GAAAAAGCTAAAAAAGTGCTGTGACAATCCGCATATTCCTTCCGCAAAACTGCGCGGGATAAAGGACTGCTACAAAATAA
AATTACGTGCGTCAGGTTTTCGCTTGGTCTATCAGGTGATTGACGAACAATTAATTATCGCTGTTGTAGCAGTGGGTAAA
CGTGAGCGCAGTGACGTTTATAATCTTGCCAGTGAAAGAATGAGATAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9178.47 Da
• Isoelectric Point: 4.2108

>AT44102 WP_001132895.1 NZ_AP027444:c46670-46419 [Escherichia coli]
MPNIILSDTSASVSELKKNPMATVSAGDGFPVAILNRNQPAFYCVPAELYEKMLDALDDQELVKLVAERSNQPLHDVDLD
KYL

Nucleotide

• Length: 252 bp

>AT44102 NZ_AP027444:c46670-46419 [Escherichia coli]
ATGCCTAACATTATTCTCAGTGATACAAGCGCCAGTGTCAGCGAGCTGAAGAAAAATCCGATGGCGACAGTCAGCGCCGG
TGATGGTTTCCCGGTCGCTATCCTGAACCGTAATCAGCCTGCTTTCTACTGTGTACCCGCAGAGCTGTACGAAAAAATGC
TCGATGCCCTGGACGATCAGGAGCTGGTTAAACTGGTAGCCGAACGCAGCAACCAACCGCTGCATGATGTGGACTTGGAT
AAATACCTATGA

Structures

Antitoxin

• AlphaFold DB: L4J0V6