Detailed information of TA system TA044100

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-DnaT
• Location: 1603..2125
• Replicon: plasmid pEC291_2
• Accession: NZ_AP027443
• Organism: Escherichia coli strain EC291 isolate EC291

Toxin (Protein)

• Gene name: relE
• Uniprot ID: U9YLU1
• Locus tag: QUE55_RS00660
• Protein ID: WP_000638823.1
• Coordinates: 1844..2125 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: U9Y7J1
• Locus tag: QUE55_RS00655
• Protein ID: WP_000121741.1
• Coordinates: 1603..1854 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QUE55_RS00640 (MUTS14_01160)	241..495	+	255	WP_001043555.1	EexN family lipoprotein	-
QUE55_RS00645 (MUTS14_01170)	508..795	+	288	WP_001032611.1	EexN family lipoprotein	-
QUE55_RS00650	868..1503	+	636	WP_015059536.1	hypothetical protein	-
QUE55_RS00655 (MUTS14_01180)	1603..1854	+	252	WP_000121741.1	hypothetical protein	Antitoxin
QUE55_RS00660 (MUTS14_01190)	1844..2125	+	282	WP_000638823.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
QUE55_RS00665 (MUTS14_01200)	2193..2492	+	300	WP_000835764.1	TrbM/KikA/MpfK family conjugal transfer protein	-
QUE55_RS00670 (MUTS14_01210)	2495..3730	+	1236	WP_015059538.1	TcpQ domain-containing protein	-
QUE55_RS00675 (MUTS14_01220)	3736..4173	+	438	WP_000539665.1	type IV pilus biogenesis protein PilM	-
QUE55_RS00680 (MUTS14_01230)	4292..4690	+	399	WP_001153665.1	hypothetical protein	-
QUE55_RS00685 (MUTS14_01240)	4711..5295	+	585	WP_001177113.1	lytic transglycosylase domain-containing protein	-
QUE55_RS00690 (MUTS14_01250)	5295..5585	+	291	WP_000865479.1	conjugal transfer protein	-
QUE55_RS00695 (MUTS14_01260)	5656..5976	+	321	WP_000362080.1	VirB3 family type IV secretion system protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	mcr-1.1	-	1..60733	60733

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-87)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10945.79 Da
• Isoelectric Point: 10.4997

>T44100 WP_000638823.1 NZ_AP027443:1844-2125 [Escherichia coli]
MIYELAFDHRALKEWQKLGHTVREQFKKKLSERLINPRVPAAKLHGHTDRYKIKLRASGYRLVYQVIDEKVVLLVISVGK
REGSDVYHMADTR

Nucleotide

• Length: 282 bp

>T44100 NZ_AP027443:1844-2125 [Escherichia coli]
ATGATTTATGAACTGGCATTTGACCACAGAGCACTGAAGGAATGGCAAAAACTCGGTCACACTGTTCGTGAGCAATTCAA
AAAGAAACTGTCAGAACGCCTGATAAATCCACGCGTCCCCGCAGCCAAGCTACATGGTCATACTGACCGTTACAAAATTA
AGCTTCGCGCATCCGGTTACAGGCTCGTTTATCAGGTAATTGATGAGAAGGTCGTTTTGCTTGTGATTTCTGTGGGAAAA
AGAGAAGGCAGCGACGTCTATCACATGGCAGATACCCGCTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9223.38 Da
• Isoelectric Point: 3.9823

>AT44100 WP_000121741.1 NZ_AP027443:1603-1854 [Escherichia coli]
MSYQILTTTAASITDLKKNPMGTVAEGEGDAVAILNRNEPAFYCVPPELYAYYRELAEDAELNAIADERMKNPDIVRVNL
DDL

Nucleotide

• Length: 252 bp

>AT44100 NZ_AP027443:1603-1854 [Escherichia coli]
ATGAGCTACCAAATTCTGACTACGACAGCAGCCAGTATTACTGACCTGAAAAAAAATCCTATGGGAACTGTAGCCGAAGG
AGAAGGGGATGCCGTCGCAATCCTGAACCGAAATGAACCAGCGTTCTACTGTGTCCCACCAGAACTTTATGCCTACTACC
GGGAACTCGCTGAAGACGCTGAACTCAACGCAATTGCTGATGAAAGAATGAAAAATCCAGACATTGTAAGGGTTAACCTG
GATGATTTATGA

Structures

Toxin

• AlphaFold DB: U9YLU1

Antitoxin

• AlphaFold DB: U9Y7J1