Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 39358..40001 Replicon plasmid pEC291_3
Accession NZ_AP027442
Organism Escherichia coli strain EC291 isolate EC291

Toxin (Protein)


Gene name vagD Uniprot ID Q84A06
Locus tag QUE55_RS00505 Protein ID WP_000754566.1
Coordinates 39358..39774 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID Q84A07
Locus tag QUE55_RS00510 Protein ID WP_001261276.1
Coordinates 39771..40001 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QUE55_RS00485 35839..36642 + 804 WP_001082320.1 aminoglycoside O-phosphotransferase APH(3'')-Ib -
QUE55_RS00490 (MUTS14_00880) 36642..37478 + 837 WP_000480972.1 aminoglycoside O-phosphotransferase APH(6)-Id -
QUE55_RS00495 (MUTS14_00890) 37585..38016 - 432 Protein_29 IS110 family transposase -
QUE55_RS00500 38298..39154 - 857 Protein_30 IS3-like element ISEc15 family transposase -
QUE55_RS00505 (MUTS14_00920) 39358..39774 - 417 WP_000754566.1 type II toxin-antitoxin system VapC family toxin Toxin
QUE55_RS00510 (MUTS14_00930) 39771..40001 - 231 WP_001261276.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
QUE55_RS00515 (MUTS14_00940) 40575..40925 + 351 WP_000493378.1 hypothetical protein -
QUE55_RS00520 (MUTS14_00950) 40976..41719 + 744 WP_000129823.1 hypothetical protein -
QUE55_RS00525 (MUTS14_00960) 41716..42492 + 777 WP_000015958.1 site-specific integrase -
QUE55_RS00530 (MUTS14_00970) 42550..42807 - 258 WP_000764641.1 hypothetical protein -
QUE55_RS00535 42936..43040 - 105 WP_032409716.1 hypothetical protein -
QUE55_RS00540 (MUTS14_00980) 43575..44441 + 867 WP_004118283.1 replication initiation protein -
QUE55_RS00545 (MUTS14_00990) 44798..44995 - 198 WP_000339856.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid blaTEM-1B / tet(B) / aph(3'')-Ib / aph(6)-Id - 1..60421 60421


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15006.52 Da        Isoelectric Point: 9.2957

>T44099 WP_000754566.1 NZ_AP027442:c39774-39358 [Escherichia coli]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAVLPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNVREFARVPGLVLEDWVK

Download         Length: 417 bp

>T44099 NZ_AP027442:c39774-39358 [Escherichia coli]
ATGAAGAAAACCTGGATGCTCGACACCAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGTGATCGCATTGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCGTCCTGCCCTGGGACCGCGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTAGCCGGGACGCCGATCGGCCCGAATGACACGGCCATTGC
CGGGCACGCCATCGCGGCGGGCGCCATCCTGGTGACGAACAATGTGAGAGAGTTTGCGCGGGTCCCGGGCCTGGTGCTGG
AAGACTGGGTGAAATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8721.74 Da        Isoelectric Point: 4.3533

>AT44099 WP_001261276.1 NZ_AP027442:c40001-39771 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Download         Length: 231 bp

>AT44099 NZ_AP027442:c40001-39771 [Escherichia coli]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGATGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGATCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A387K1G3

Antitoxin

Source ID Structure
AlphaFold DB A0A387K023

References