Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | relBE/ParE-DnaT |
| Location | 40175..40697 | Replicon | plasmid pEC291_4 |
| Accession | NZ_AP027441 | ||
| Organism | Escherichia coli strain EC291 isolate EC291 | ||
Toxin (Protein)
| Gene name | relE | Uniprot ID | G3CAN5 |
| Locus tag | QUE55_RS00265 | Protein ID | WP_000220560.1 |
| Coordinates | 40175..40456 (-) | Length | 94 a.a. |
Antitoxin (Protein)
| Gene name | relB | Uniprot ID | G3CAG1 |
| Locus tag | QUE55_RS00270 | Protein ID | WP_000121743.1 |
| Coordinates | 40446..40697 (-) | Length | 84 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| QUE55_RS00220 (MUTS14_00410) | 36023..37189 | - | 1167 | WP_000539530.1 | MobP1 family relaxase | - |
| QUE55_RS00225 (MUTS14_00420) | 37192..37737 | - | 546 | WP_015060502.1 | DNA distortion polypeptide 1 | - |
| QUE55_RS00230 (MUTS14_00430) | 38063..38335 | - | 273 | WP_000160399.1 | hypothetical protein | - |
| QUE55_RS00235 (MUTS14_00440) | 38348..38497 | - | 150 | WP_000003880.1 | hypothetical protein | - |
| QUE55_RS00240 (MUTS14_00450) | 38567..38746 | - | 180 | WP_000439078.1 | hypothetical protein | - |
| QUE55_RS00245 (MUTS14_00460) | 38789..39118 | - | 330 | WP_000866648.1 | hypothetical protein | - |
| QUE55_RS00250 (MUTS14_00470) | 39211..39426 | - | 216 | WP_001180116.1 | hypothetical protein | - |
| QUE55_RS00255 (MUTS14_00480) | 39416..39661 | - | 246 | WP_000356546.1 | hypothetical protein | - |
| QUE55_RS00260 (MUTS14_00490) | 39706..40029 | - | 324 | WP_001181903.1 | hypothetical protein | - |
| QUE55_RS00265 (MUTS14_00500) | 40175..40456 | - | 282 | WP_000220560.1 | type II toxin-antitoxin system RelE/ParE family toxin | Toxin |
| QUE55_RS00270 (MUTS14_00510) | 40446..40697 | - | 252 | WP_000121743.1 | plasmid stabilization protein | Antitoxin |
| QUE55_RS00275 (MUTS14_00520) | 41931..42767 | + | 837 | WP_001050931.1 | RepB family plasmid replication initiator protein | - |
| QUE55_RS00280 (MUTS14_00530) | 42807..43253 | + | 447 | WP_001074386.1 | hypothetical protein | - |
| QUE55_RS00285 (MUTS14_00540) | 43390..43743 | + | 354 | WP_000876434.1 | DNA distortion polypeptide 3 | - |
| QUE55_RS00290 | 43803..44201 | - | 399 | Protein_57 | IS5-like element IS903B family transposase | - |
| QUE55_RS00295 | 44349..44435 | - | 87 | Protein_58 | hypothetical protein | - |
| QUE55_RS00300 (MUTS14_00560) | 44488..45192 | - | 705 | WP_001067858.1 | IS6-like element IS26 family transposase | - |
| QUE55_RS00305 | 45296..45526 | + | 231 | WP_074135294.1 | helix-turn-helix domain-containing protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Non-Mobilizable plasmid | floR | mrkF / mrkD / mrkC / mrkB / mrkA | 1..51942 | 51942 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 94 a.a. Molecular weight: 11005.83 Da Isoelectric Point: 10.5938
>T44098 WP_000220560.1 NZ_AP027441:c40456-40175 [Escherichia coli]
MTYELAFDRRALKEWQKLGHTIREQFKKKLAERLENPRVPAARLHGHADRYKIKLRASGYRLVYQVIDEKVVLLVIAVGR
RESSEVYQIADLR
MTYELAFDRRALKEWQKLGHTIREQFKKKLAERLENPRVPAARLHGHADRYKIKLRASGYRLVYQVIDEKVVLLVIAVGR
RESSEVYQIADLR
Download Length: 282 bp
>T44098 NZ_AP027441:c40456-40175 [Escherichia coli]
ATGACCTATGAACTGGCTTTTGACCGTAGAGCACTGAAGGAATGGCAGAAACTCGGCCACACCATCCGTGAACAATTCAA
AAAGAAACTGGCAGAACGGCTGGAAAATCCACGCGTACCCGCAGCCCGGTTACATGGTCATGCTGATCGCTATAAAATCA
AACTTCGTGCATCTGGCTACAGACTTGTATACCAAGTCATTGATGAGAAAGTCGTTTTACTCGTTATTGCCGTTGGAAGA
AGGGAAAGCAGCGAAGTCTATCAGATCGCAGATTTGCGCTAA
ATGACCTATGAACTGGCTTTTGACCGTAGAGCACTGAAGGAATGGCAGAAACTCGGCCACACCATCCGTGAACAATTCAA
AAAGAAACTGGCAGAACGGCTGGAAAATCCACGCGTACCCGCAGCCCGGTTACATGGTCATGCTGATCGCTATAAAATCA
AACTTCGTGCATCTGGCTACAGACTTGTATACCAAGTCATTGATGAGAAAGTCGTTTTACTCGTTATTGCCGTTGGAAGA
AGGGAAAGCAGCGAAGTCTATCAGATCGCAGATTTGCGCTAA
Antitoxin
Download Length: 84 a.a. Molecular weight: 9194.43 Da Isoelectric Point: 4.1683
>AT44098 WP_000121743.1 NZ_AP027441:c40697-40446 [Escherichia coli]
MSYQILTTTAASITDLKKNPMGTVAEGEGDAVAILNRNEPAFYCVPPKLYAYYRELAEDAELNAVADERMKNPEIVKVNL
DDL
MSYQILTTTAASITDLKKNPMGTVAEGEGDAVAILNRNEPAFYCVPPKLYAYYRELAEDAELNAVADERMKNPEIVKVNL
DDL
Download Length: 252 bp
>AT44098 NZ_AP027441:c40697-40446 [Escherichia coli]
ATGAGCTATCAAATTCTGACAACCACAGCGGCCAGTATTACTGACCTGAAAAAAAATCCTATGGGAACCGTAGCTGAAGG
TGAAGGGGACGCTGTTGCGATCCTGAACCGAAACGAACCGGCGTTCTATTGCGTTCCACCAAAACTTTACGCCTACTATC
GGGAACTCGCTGAAGATGCTGAGTTAAACGCTGTTGCTGATGAGCGCATGAAAAACCCGGAAATTGTGAAGGTTAACCTG
GATGACCTATGA
ATGAGCTATCAAATTCTGACAACCACAGCGGCCAGTATTACTGACCTGAAAAAAAATCCTATGGGAACCGTAGCTGAAGG
TGAAGGGGACGCTGTTGCGATCCTGAACCGAAACGAACCGGCGTTCTATTGCGTTCCACCAAAACTTTACGCCTACTATC
GGGAACTCGCTGAAGATGCTGAGTTAAACGCTGTTGCTGATGAGCGCATGAAAAACCCGGAAATTGTGAAGGTTAACCTG
GATGACCTATGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | G3CAN5 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A3S5I301 |