Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA-HicB
Location 3363274..3363886 Replicon chromosome
Accession NZ_AP027440
Organism Escherichia coli strain EC556 isolate EC556

Toxin (Protein)


Gene name hicA Uniprot ID U9YXE2
Locus tag QUE77_RS17960 Protein ID WP_000833473.1
Coordinates 3363274..3363459 (+) Length 62 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A1V3VHB3
Locus tag QUE77_RS17965 Protein ID WP_000499746.1
Coordinates 3363476..3363886 (+) Length 137 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QUE77_RS17945 (3358738) 3358738..3360033 + 1296 WP_025210981.1 Fic family protein -
QUE77_RS17950 (3360141) 3360141..3361679 + 1539 WP_025210980.1 aldehyde dehydrogenase AldB -
QUE77_RS17955 (3361720) 3361720..3362799 - 1080 WP_000061477.1 type I restriction enzyme HsdR N-terminal domain-containing protein -
QUE77_RS17960 (3363274) 3363274..3363459 + 186 WP_000833473.1 type II toxin-antitoxin system HicA family toxin Toxin
QUE77_RS17965 (3363476) 3363476..3363886 + 411 WP_000499746.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
QUE77_RS17970 (3363958) 3363958..3365922 - 1965 WP_096098550.1 glycoside hydrolase family 127 protein -
QUE77_RS17975 (3365933) 3365933..3367333 - 1401 WP_096098549.1 MFS transporter -
QUE77_RS17980 (3367559) 3367559..3368374 + 816 WP_000891838.1 AraC family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(4-59)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(3-129)


Sequences

Toxin        


Download         Length: 62 a.a.        Molecular weight: 6800.89 Da        Isoelectric Point: 11.7053

>T44094 WP_000833473.1 NZ_AP027440:3363274-3363459 [Escherichia coli]
VKSADVIAILKQHGWEHIRTRGSHHQFRHPVHPGLVTVPHPKKDIKPGTLAQIGRQAGLTF

Download         Length: 186 bp

>T44094 NZ_AP027440:3363274-3363459 [Escherichia coli]
GTGAAAAGTGCAGACGTGATCGCCATCCTGAAGCAGCATGGCTGGGAACATATTCGAACCCGTGGCAGTCATCATCAGTT
CCGCCACCCTGTTCATCCGGGTCTGGTGACGGTTCCGCACCCTAAAAAAGATATTAAGCCCGGTACGCTGGCGCAAATTG
GGCGTCAGGCTGGTTTAACATTTTAA

Antitoxin


Download         Length: 137 a.a.        Molecular weight: 15290.22 Da        Isoelectric Point: 4.5486

>AT44094 WP_000499746.1 NZ_AP027440:3363476-3363886 [Escherichia coli]
MFYPAYIHSDPDGSASGFFPDVPGCYFAGDTLDYAFQDARSALVAHFETLCEMNEELPLPGSVETHLVQRAQDFIGGQWL
LVDINMNQFEGRAERINITMPKRLLNKIDTYVRNNPDYANRSAFLAEAARRVLPGV

Download         Length: 411 bp

>AT44094 NZ_AP027440:3363476-3363886 [Escherichia coli]
ATGTTTTATCCCGCCTATATCCACAGTGATCCTGACGGCAGTGCCAGCGGTTTTTTCCCTGATGTTCCAGGCTGTTATTT
TGCTGGTGATACGCTGGACTACGCTTTCCAGGATGCGCGAAGTGCACTGGTCGCGCACTTCGAAACCTTATGCGAGATGA
ATGAAGAGTTGCCGCTTCCAGGCTCTGTGGAAACACATCTGGTCCAGCGTGCGCAGGACTTTATTGGCGGGCAATGGTTG
CTGGTGGATATCAATATGAATCAGTTCGAAGGTCGGGCTGAACGTATCAATATTACGATGCCAAAAAGATTACTGAACAA
GATTGATACCTACGTGCGCAATAACCCAGACTATGCCAACAGAAGTGCGTTTCTGGCGGAAGCTGCCCGGCGTGTATTGC
CGGGCGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9YXE2

Antitoxin

Source ID Structure
AlphaFold DB A0A1V3VHB3

References