Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 2703517..2704014 Replicon chromosome
Accession NZ_AP027440
Organism Escherichia coli strain EC556 isolate EC556

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag QUE77_RS14940 Protein ID WP_001173343.1
Coordinates 2703517..2703690 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag QUE77_RS14945 Protein ID WP_000398619.1
Coordinates 2703718..2704014 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QUE77_RS14930 (2700187) 2700187..2701644 + 1458 WP_001550473.1 dipeptide/tripeptide permease DtpC -
QUE77_RS14935 (2701881) 2701881..2703398 + 1518 WP_001295074.1 lysine--tRNA ligase -
QUE77_RS14940 (2703517) 2703517..2703690 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
QUE77_RS14945 (2703718) 2703718..2704014 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
QUE77_RS14950 (2704242) 2704242..2704514 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
QUE77_RS14955 (2704526) 2704526..2704756 - 231 WP_001550470.1 4Fe-4S mono-cluster protein YjdI -
QUE77_RS14960 (2704936) 2704936..2706567 + 1632 WP_001216482.1 sensor histidine kinase -
QUE77_RS14965 (2706564) 2706564..2707283 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T44092 WP_001173343.1 NZ_AP027440:c2703690-2703517 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T44092 NZ_AP027440:c2703690-2703517 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT44092 WP_000398619.1 NZ_AP027440:c2704014-2703718 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT44092 NZ_AP027440:c2704014-2703718 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GACTTTGACCTCAACATTATGACAGTTGATGATTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References