Detailed information of TA system TA044091

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 2690956..2691791
• Replicon: chromosome
• Accession: NZ_AP027440
• Organism: Escherichia coli strain EC556 isolate EC556

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A7W4PV54
• Locus tag: QUE77_RS14885
• Protein ID: WP_000854821.1
• Coordinates: 2691414..2691791 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: B7MK11
• Locus tag: QUE77_RS14880
• Protein ID: WP_001285610.1
• Coordinates: 2690956..2691324 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QUE77_RS14855 (2688361)	2688361..2688594	+	234	WP_001117568.1	DUF905 family protein	-
QUE77_RS14860 (2688684)	2688684..2689502	+	819	WP_001175136.1	DUF932 domain-containing protein	-
QUE77_RS14865 (2689594)	2689594..2690076	+	483	WP_000206657.1	antirestriction protein	-
QUE77_RS14870 (2690092)	2690092..2690568	+	477	WP_001384108.1	RadC family protein	-
QUE77_RS14875 (2690655)	2690655..2690876	+	222	WP_000692350.1	DUF987 domain-containing protein	-
QUE77_RS14880 (2690956)	2690956..2691324	+	369	WP_001285610.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
QUE77_RS14885 (2691414)	2691414..2691791	+	378	WP_000854821.1	type IV toxin-antitoxin system cytoskeleton-binding toxin CbtA	Toxin
QUE77_RS14890 (2691788)	2691788..2691985	+	198	Protein_2657	DUF5983 family protein	-
QUE77_RS14895 (2692013)	2692013..2692210	+	198	WP_085949090.1	DUF957 domain-containing protein	-
QUE77_RS14900 (2692295)	2692295..2693137	+	843	WP_001280493.1	DUF4942 domain-containing protein	-
QUE77_RS14905 (2693886)	2693886..2695118	+	1233	Protein_2660	lysine decarboxylation/transport transcriptional activator CadC	-
QUE77_RS14915 (2695890)	2695890..2696201	+	312	Protein_2662	transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (19-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13930.95 Da
• Isoelectric Point: 8.5163

>T44091 WP_000854821.1 NZ_AP027440:2691414-2691791 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPGAKQ

Nucleotide

• Length: 378 bp

>T44091 NZ_AP027440:2691414-2691791 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCACGCCCGTCTCCGGTTGAAATCTGGCAGACACTGCTCAC
CCGACTGCTGGATCAGCACTACGGCCTTACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGCGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTACGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGTTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCACGCCGGGCAACCGGCCTGATGACACGCGA
CAACTACAGAACGGTAAATAACATTACCCTGGGTAAACATCCGGGGGCGAAACAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13606.39 Da
• Isoelectric Point: 6.0618

>AT44091 WP_001285610.1 NZ_AP027440:2690956-2691324 [Escherichia coli]
VSDTLPGTTLPDDNNDRTWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADSYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYMAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT44091 NZ_AP027440:2690956-2691324 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACGCTTCCCGACGACAATAACGACCGCACCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTTGGTGCCCGTCTGGTACAGGAAGGCAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGGCGGT
TCAGCGACGCGGATTCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCAGTTG
TGGCTACGTTTATATGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A7W4PV54