Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 2499771..2500347 Replicon chromosome
Accession NZ_AP027440
Organism Escherichia coli strain EC556 isolate EC556

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag QUE77_RS13895 Protein ID WP_001297643.1
Coordinates 2500060..2500347 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A080I7H7
Locus tag QUE77_RS13890 Protein ID WP_000063160.1
Coordinates 2499771..2500073 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QUE77_RS13875 (2496367) 2496367..2498517 + 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
QUE77_RS13880 (2498567) 2498567..2498770 + 204 WP_000467859.1 YbdD/YjiX family protein -
QUE77_RS13885 (2498781) 2498781..2499737 + 957 WP_001297640.1 GTPase -
QUE77_RS13890 (2499771) 2499771..2500073 - 303 WP_000063160.1 BrnA antitoxin family protein Antitoxin
QUE77_RS13895 (2500060) 2500060..2500347 - 288 WP_001297643.1 BrnT family toxin Toxin
QUE77_RS13905 (2501300) 2501300..2501395 + 96 Protein_2465 dienelactone hydrolase family protein -
QUE77_RS13910 (2501541) 2501541..2502773 + 1233 WP_024186283.1 multidrug efflux MFS transporter MdtM -
QUE77_RS13915 (2502814) 2502814..2504094 + 1281 WP_001037400.1 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 2500546..2500839 293


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T44090 WP_001297643.1 NZ_AP027440:c2500347-2500060 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T44090 NZ_AP027440:c2500347-2500060 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGAAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTATTGTTTGATGATCCACAACATTTGTCACAGCAAGAACGCATAGAAAACGGTGAGTATCGCTGGCAGACAATCGGCC
TGGTTTACGGCATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAATCGCTATGAGCATGGTTAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11552.01 Da        Isoelectric Point: 9.4646

>AT44090 WP_000063160.1 NZ_AP027440:c2500073-2499771 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALTNKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT44090 NZ_AP027440:c2500073-2499771 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCGCTGACGAA
TAAATCCGACGAAGATATTGATTACAGTGATATCCCACCTTCCAGCGATGAGCAATGGTCTAACGCCGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGTTGAAGCGACCAGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A080I7H7

References