Detailed information of TA system TA044086

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-HTH_XRE
• Location: 1491669..1492374
• Replicon: chromosome
• Accession: NZ_AP027440
• Organism: Escherichia coli strain EC556 isolate EC556

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A3A6SJV3
• Locus tag: QUE77_RS09015
• Protein ID: WP_063085620.1
• Coordinates: 1491669..1492055 (+)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: QUE77_RS09020
• Protein ID: WP_001280945.1
• Coordinates: 1492045..1492374 (+)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QUE77_RS08995 (1487673)	1487673..1488299	+	627	WP_001314584.1	glutathione S-transferase GstB	-
QUE77_RS09000 (1488296)	1488296..1489411	-	1116	WP_063085618.1	aldose sugar dehydrogenase YliI	-
QUE77_RS09005 (1489522)	1489522..1489905	-	384	WP_000497137.1	biofilm formation regulator BssR	-
QUE77_RS09010 (1490118)	1490118..1491443	+	1326	WP_000049367.1	30S ribosomal protein S12 methylthiotransferase RimO	-
QUE77_RS09015 (1491669)	1491669..1492055	+	387	WP_063085620.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
QUE77_RS09020 (1492045)	1492045..1492374	+	330	WP_001280945.1	DNA-binding transcriptional regulator	Antitoxin
QUE77_RS09025 (1492444)	1492444..1493772	-	1329	WP_000086873.1	GGDEF domain-containing protein	-
QUE77_RS09030 (1493780)	1493780..1496128	-	2349	WP_021523051.1	EAL domain-containing protein	-
QUE77_RS09035 (1496306)	1496306..1497217	-	912	WP_001236019.1	glutathione ABC transporter permease GsiD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-124)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-94)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14280.45 Da
• Isoelectric Point: 9.9296

>T44086 WP_063085620.1 NZ_AP027440:1491669-1492055 [Escherichia coli]
MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVIKKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSG
LSSKGTKEIEDDELAAYKKMANAFLAFSTKQIEDLIETGFLIEVKNER

Nucleotide

• Length: 387 bp

>T44086 NZ_AP027440:1491669-1492055 [Escherichia coli]
ATGGGTGTGTATAAGGCAAGACGATTCAGCCAAAGCACGAAAAAACTTGGTATTCACGATAAAGTTCTCATGGCTGCCGC
TGAAGAAGTGATGCAGGGCATATGGGAGGCTGATTTAGGTAGCGGTGTCATCAAAAAACGTTTACCCCTCCAACAAGGAA
AGAGTGGTGGAGCCAGAACAATTATCTTTTTTAAATCAGCAAATCATGTGTTTTTTTATGATGGTTGGTCCAAGAGTGGT
CTAAGCAGTAAAGGAACCAAAGAAATCGAAGATGATGAACTGGCGGCCTACAAAAAAATGGCAAATGCATTTCTGGCCTT
TAGCACTAAGCAAATAGAGGATTTGATCGAAACTGGCTTTTTGATTGAGGTGAAAAATGAGCGATAA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11982.08 Da
• Isoelectric Point: 10.0101

>AT44086 WP_001280945.1 NZ_AP027440:1492045-1492374 [Escherichia coli]
MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAKVKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSK
WERNEIKPSGPALRILNTLAIKGPEVFAK

Nucleotide

• Length: 330 bp

>AT44086 NZ_AP027440:1492045-1492374 [Escherichia coli]
ATGAGCGATAAATTACAAATGATGCTGGAAGATGCAAAGGAACTGAATGCACTGGGCCTGATGTCTGATAGTGAAGTTGA
TGCCATTGCCGTCATGGTCAAAGCTCGTGAAGTCAGCGCCCGTATAGCCAAAGTCAAATCAATGACGGGTGAAGAGATCA
AAGCCATCCGCACCCGATACGGCATGTCACAATCCGTGCTGGCACATACAATGGGAATGTCAAAAGAAAGTGTCTCGAAA
TGGGAACGTAATGAAATAAAACCGAGCGGCCCGGCATTACGTATTTTGAATACATTGGCAATAAAAGGGCCGGAAGTTTT
TGCAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A3A6SJV3