Detailed information of TA system TA044084

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 780725..781363
• Replicon: chromosome
• Accession: NZ_AP027440
• Organism: Escherichia coli strain EC556 isolate EC556

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A3A6S5Q3
• Locus tag: QUE77_RS05445
• Protein ID: WP_063085535.1
• Coordinates: 781187..781363 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: QUE77_RS05440
• Protein ID: WP_001270286.1
• Coordinates: 780725..781141 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QUE77_RS05420 (775877)	775877..776818	-	942	WP_063085538.1	ABC transporter permease	-
QUE77_RS05425 (776819)	776819..777832	-	1014	WP_063085537.1	ABC transporter ATP-binding protein	-
QUE77_RS05430 (777850)	777850..778995	-	1146	WP_000047450.1	ABC transporter substrate-binding protein	-
QUE77_RS05435 (779240)	779240..780646	-	1407	WP_063085536.1	PLP-dependent aminotransferase family protein	-
QUE77_RS05440 (780725)	780725..781141	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
QUE77_RS05445 (781187)	781187..781363	-	177	WP_063085535.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
QUE77_RS05450 (781585)	781585..781815	+	231	WP_000494244.1	YncJ family protein	-
QUE77_RS05455 (781907)	781907..783868	-	1962	WP_063085534.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
QUE77_RS05460 (783941)	783941..784477	-	537	WP_000429507.1	DNA-binding transcriptional regulator SutR	-
QUE77_RS05465 (784569)	784569..785740	+	1172	Protein_812	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6751.78 Da
• Isoelectric Point: 11.5666

>T44084 WP_063085535.1 NZ_AP027440:c781363-781187 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFQGRRSVMPRHPNDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T44084 NZ_AP027440:c781363-781187 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTGGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCAAGGGCGACGCAGTGTCATGCCGCGTCACCCCAATGATGAGATAAAAGAACCATTGCGTAAAGCAATCCTGAAGC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT44084 WP_001270286.1 NZ_AP027440:c781141-780725 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT44084 NZ_AP027440:c781141-780725 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTGCCTTTGAGCGTCGCCTCTAAAGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAAAT
TACTCGCTTATTTAACTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTTGCGGCTAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• AlphaFold DB: A0A3A6S5Q3