Detailed information of TA system TA044068

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 63828..64387
• Replicon: plasmid pEC556_2
• Accession: NZ_AP027438
• Organism: Escherichia coli strain EC556 isolate EC556

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1PMX5
• Locus tag: QUE77_RS00800
• Protein ID: WP_000421257.1
• Coordinates: 64112..64387 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: C1J8K5
• Locus tag: QUE77_RS00795
• Protein ID: WP_001178089.1
• Coordinates: 63828..64112 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QUE77_RS00760	59465..59716	+	252	WP_001372275.1	hypothetical protein	-
QUE77_RS00765 (MUTS11_01390)	59915..60376	+	462	WP_001233868.1	thermonuclease family protein	-
QUE77_RS00770 (MUTS11_01400)	60422..60631	+	210	WP_001610317.1	hemolysin expression modulator Hha	-
QUE77_RS00775 (MUTS11_01410)	60669..61259	+	591	WP_000766807.1	DUF2726 domain-containing protein	-
QUE77_RS00780 (MUTS11_01420)	61499..61753	+	255	WP_000083830.1	replication regulatory protein RepA	-
QUE77_RS00785	61992..62066	+	75	WP_001365705.1	RepA leader peptide Tap	-
QUE77_RS00790 (MUTS11_01430)	62059..62916	+	858	WP_000130988.1	incFII family plasmid replication initiator RepA	-
QUE77_RS00795 (MUTS11_01440)	63828..64112	+	285	WP_001178089.1	ribbon-helix-helix domain-containing protein	Antitoxin
QUE77_RS00800 (MUTS11_01450)	64112..64387	+	276	WP_000421257.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
QUE77_RS00805 (MUTS11_01460)	64671..65858	-	1188	WP_286338237.1	IS91-like element IS91 family transposase	-
QUE77_RS00810 (MUTS11_01470)	65858..66223	-	366	WP_000124096.1	hypothetical protein	-
QUE77_RS00815 (MUTS11_01480)	66498..67685	-	1188	WP_000937591.1	IS91 family transposase	-
QUE77_RS00820 (MUTS11_01490)	67685..68050	-	366	WP_000091308.1	hypothetical protein	-
QUE77_RS00825 (MUTS11_01510)	68473..68946	-	474	WP_001122138.1	type II toxin-antitoxin system PemK/MazF family toxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	mcr-5.1	hlyD / hlyB / hlyA / hlyC	1..72582	72582

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (14-51)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10749.45 Da
• Isoelectric Point: 9.9324

>T44068 WP_000421257.1 NZ_AP027438:64112-64387 [Escherichia coli]
MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIY
VLRLWHTRENR

Nucleotide

• Length: 276 bp

>T44068 NZ_AP027438:64112-64387 [Escherichia coli]
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTGGCCAGTAAACCTGC
TGCCGCCAGAACGGTACAGTCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAACTTAATGGCCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10456.86 Da
• Isoelectric Point: 5.6558

>AT44068 WP_001178089.1 NZ_AP027438:63828-64112 [Escherichia coli]
IQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDNPLPVPR

Nucleotide

• Length: 285 bp

>AT44068 NZ_AP027438:63828-64112 [Escherichia coli]
ATACAAATGAAAAACAATACCGCACAGGCAACAAAAGTCATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGGCTGGAACGCTCCCGGGGCTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAAGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATCGACCATCAGGCTGTACAGGCCTGG
GCGGACAGTCTCAGTACTGACAATCCGTTACCGGTGCCCCGCTGA

Structures

Toxin

• AlphaFold DB: S1PMX5

Antitoxin

• AlphaFold DB: A0A731IHA8