Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 4146259..4146913 Replicon chromosome
Accession NZ_AP027436
Organism Escherichia coli strain EC571 isolate EC571

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag QUE57_RS21735 Protein ID WP_000244781.1
Coordinates 4146506..4146913 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag QUE57_RS21730 Protein ID WP_000354046.1
Coordinates 4146259..4146525 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QUE57_RS21710 (4142347) 4142347..4143780 - 1434 WP_063085817.1 6-phospho-beta-glucosidase BglA -
QUE57_RS21715 (4143825) 4143825..4144136 + 312 WP_001182952.1 N(4)-acetylcytidine aminohydrolase -
QUE57_RS21720 (4144300) 4144300..4144959 + 660 WP_000250269.1 hemolysin III family protein -
QUE57_RS21725 (4145036) 4145036..4146016 - 981 WP_000886050.1 tRNA-modifying protein YgfZ -
QUE57_RS21730 (4146259) 4146259..4146525 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
QUE57_RS21735 (4146506) 4146506..4146913 + 408 WP_000244781.1 protein YgfX Toxin
QUE57_RS21740 (4146953) 4146953..4147474 - 522 WP_001055888.1 flavodoxin FldB -
QUE57_RS21745 (4147586) 4147586..4148482 + 897 WP_000806652.1 site-specific tyrosine recombinase XerD -
QUE57_RS21750 (4148507) 4148507..4149217 + 711 WP_000715213.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
QUE57_RS21755 (4149223) 4149223..4150956 + 1734 WP_000813177.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T44066 WP_000244781.1 NZ_AP027436:4146506-4146913 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T44066 NZ_AP027436:4146506-4146913 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATCTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATCCACGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGCATTAATGCTCGCCAGGGGGAAATTCGCCTGTTGATGGACGGGCGTTTGCGCTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGACAGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATATTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT44066 WP_000354046.1 NZ_AP027436:4146259-4146525 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT44066 NZ_AP027436:4146259-4146525 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAGTGTGACGATCCCGATC
TGTTTAACTGGCTGATGAACCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References