Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA-HicB
Location 3361556..3362168 Replicon chromosome
Accession NZ_AP027436
Organism Escherichia coli strain EC571 isolate EC571

Toxin (Protein)


Gene name hicA Uniprot ID U9YXE2
Locus tag QUE57_RS17935 Protein ID WP_000833473.1
Coordinates 3361556..3361741 (+) Length 62 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A1V3VHB3
Locus tag QUE57_RS17940 Protein ID WP_000499746.1
Coordinates 3361758..3362168 (+) Length 137 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QUE57_RS17920 (3357020) 3357020..3358315 + 1296 WP_025210981.1 Fic family protein -
QUE57_RS17925 (3358423) 3358423..3359961 + 1539 WP_025210980.1 aldehyde dehydrogenase AldB -
QUE57_RS17930 (3360002) 3360002..3361081 - 1080 WP_000061477.1 type I restriction enzyme HsdR N-terminal domain-containing protein -
QUE57_RS17935 (3361556) 3361556..3361741 + 186 WP_000833473.1 type II toxin-antitoxin system HicA family toxin Toxin
QUE57_RS17940 (3361758) 3361758..3362168 + 411 WP_000499746.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
QUE57_RS17945 (3362240) 3362240..3364204 - 1965 WP_096098550.1 glycoside hydrolase family 127 protein -
QUE57_RS17950 (3364215) 3364215..3365615 - 1401 WP_096098549.1 MFS transporter -
QUE57_RS17955 (3365841) 3365841..3366656 + 816 WP_000891838.1 AraC family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(4-59)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(3-129)


Sequences

Toxin        


Download         Length: 62 a.a.        Molecular weight: 6800.89 Da        Isoelectric Point: 11.7053

>T44064 WP_000833473.1 NZ_AP027436:3361556-3361741 [Escherichia coli]
VKSADVIAILKQHGWEHIRTRGSHHQFRHPVHPGLVTVPHPKKDIKPGTLAQIGRQAGLTF

Download         Length: 186 bp

>T44064 NZ_AP027436:3361556-3361741 [Escherichia coli]
GTGAAAAGTGCAGACGTGATCGCCATCCTGAAGCAGCATGGCTGGGAACATATTCGAACCCGTGGCAGTCATCATCAGTT
CCGCCACCCTGTTCATCCGGGTCTGGTGACGGTTCCGCACCCTAAAAAAGATATTAAGCCCGGTACGCTGGCGCAAATTG
GGCGTCAGGCTGGTTTAACATTTTAA

Antitoxin


Download         Length: 137 a.a.        Molecular weight: 15290.22 Da        Isoelectric Point: 4.5486

>AT44064 WP_000499746.1 NZ_AP027436:3361758-3362168 [Escherichia coli]
MFYPAYIHSDPDGSASGFFPDVPGCYFAGDTLDYAFQDARSALVAHFETLCEMNEELPLPGSVETHLVQRAQDFIGGQWL
LVDINMNQFEGRAERINITMPKRLLNKIDTYVRNNPDYANRSAFLAEAARRVLPGV

Download         Length: 411 bp

>AT44064 NZ_AP027436:3361758-3362168 [Escherichia coli]
ATGTTTTATCCCGCCTATATCCACAGTGATCCTGACGGCAGTGCCAGCGGTTTTTTCCCTGATGTTCCAGGCTGTTATTT
TGCTGGTGATACGCTGGACTACGCTTTCCAGGATGCGCGAAGTGCACTGGTCGCGCACTTCGAAACCTTATGCGAGATGA
ATGAAGAGTTGCCGCTTCCAGGCTCTGTGGAAACACATCTGGTCCAGCGTGCGCAGGACTTTATTGGCGGGCAATGGTTG
CTGGTGGATATCAATATGAATCAGTTCGAAGGTCGGGCTGAACGTATCAATATTACGATGCCAAAAAGATTACTGAACAA
GATTGATACCTACGTGCGCAATAACCCAGACTATGCCAACAGAAGTGCGTTTCTGGCGGAAGCTGCCCGGCGTGTATTGC
CGGGCGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9YXE2

Antitoxin

Source ID Structure
AlphaFold DB A0A1V3VHB3

References