Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 2701799..2702296 Replicon chromosome
Accession NZ_AP027436
Organism Escherichia coli strain EC571 isolate EC571

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag QUE57_RS14915 Protein ID WP_001173343.1
Coordinates 2701799..2701972 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag QUE57_RS14920 Protein ID WP_000398619.1
Coordinates 2702000..2702296 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QUE57_RS14905 (2698469) 2698469..2699926 + 1458 WP_001550473.1 dipeptide/tripeptide permease DtpC -
QUE57_RS14910 (2700163) 2700163..2701680 + 1518 WP_001295074.1 lysine--tRNA ligase -
QUE57_RS14915 (2701799) 2701799..2701972 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
QUE57_RS14920 (2702000) 2702000..2702296 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
QUE57_RS14925 (2702524) 2702524..2702796 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
QUE57_RS14930 (2702808) 2702808..2703038 - 231 WP_001550470.1 4Fe-4S mono-cluster protein YjdI -
QUE57_RS14935 (2703218) 2703218..2704849 + 1632 WP_001216482.1 sensor histidine kinase -
QUE57_RS14940 (2704846) 2704846..2705565 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T44062 WP_001173343.1 NZ_AP027436:c2701972-2701799 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T44062 NZ_AP027436:c2701972-2701799 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT44062 WP_000398619.1 NZ_AP027436:c2702296-2702000 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT44062 NZ_AP027436:c2702296-2702000 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GACTTTGACCTCAACATTATGACAGTTGATGATTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References