Detailed information of TA system TA044061

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 2689238..2690073
• Replicon: chromosome
• Accession: NZ_AP027436
• Organism: Escherichia coli strain EC571 isolate EC571

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A7W4PV54
• Locus tag: QUE57_RS14860
• Protein ID: WP_000854821.1
• Coordinates: 2689696..2690073 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: B7MK11
• Locus tag: QUE57_RS14855
• Protein ID: WP_001285610.1
• Coordinates: 2689238..2689606 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QUE57_RS14830 (2686643)	2686643..2686876	+	234	WP_001117568.1	DUF905 family protein	-
QUE57_RS14835 (2686966)	2686966..2687784	+	819	WP_001175136.1	DUF932 domain-containing protein	-
QUE57_RS14840 (2687876)	2687876..2688358	+	483	WP_000206657.1	antirestriction protein	-
QUE57_RS14845 (2688374)	2688374..2688850	+	477	WP_001384108.1	RadC family protein	-
QUE57_RS14850 (2688937)	2688937..2689158	+	222	WP_000692350.1	DUF987 domain-containing protein	-
QUE57_RS14855 (2689238)	2689238..2689606	+	369	WP_001285610.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
QUE57_RS14860 (2689696)	2689696..2690073	+	378	WP_000854821.1	type IV toxin-antitoxin system cytoskeleton-binding toxin CbtA	Toxin
QUE57_RS14865 (2690070)	2690070..2690267	+	198	Protein_2655	DUF5983 family protein	-
QUE57_RS14870 (2690295)	2690295..2690492	+	198	WP_085949090.1	DUF957 domain-containing protein	-
QUE57_RS14875 (2690577)	2690577..2691419	+	843	WP_001280493.1	DUF4942 domain-containing protein	-
QUE57_RS14880 (2692168)	2692168..2693400	+	1233	Protein_2658	lysine decarboxylation/transport transcriptional activator CadC	-
QUE57_RS14890 (2694172)	2694172..2694483	+	312	Protein_2660	transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (19-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13930.95 Da
• Isoelectric Point: 8.5163

>T44061 WP_000854821.1 NZ_AP027436:2689696-2690073 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPGAKQ

Nucleotide

• Length: 378 bp

>T44061 NZ_AP027436:2689696-2690073 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCACGCCCGTCTCCGGTTGAAATCTGGCAGACACTGCTCAC
CCGACTGCTGGATCAGCACTACGGCCTTACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGCGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTACGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGTTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCACGCCGGGCAACCGGCCTGATGACACGCGA
CAACTACAGAACGGTAAATAACATTACCCTGGGTAAACATCCGGGGGCGAAACAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13606.39 Da
• Isoelectric Point: 6.0618

>AT44061 WP_001285610.1 NZ_AP027436:2689238-2689606 [Escherichia coli]
VSDTLPGTTLPDDNNDRTWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADSYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYMAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT44061 NZ_AP027436:2689238-2689606 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACGCTTCCCGACGACAATAACGACCGCACCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTTGGTGCCCGTCTGGTACAGGAAGGCAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGGCGGT
TCAGCGACGCGGATTCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCAGTTG
TGGCTACGTTTATATGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A7W4PV54