Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1939594..1940212 Replicon chromosome
Accession NZ_AP027436
Organism Escherichia coli strain EC571 isolate EC571

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag QUE57_RS11220 Protein ID WP_001291435.1
Coordinates 1939994..1940212 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag QUE57_RS11215 Protein ID WP_000344800.1
Coordinates 1939594..1939968 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QUE57_RS11205 (1935188) 1935188..1938337 + 3150 WP_001132480.1 efflux RND transporter permease AcrB -
QUE57_RS11210 (1938457) 1938457..1938915 - 459 WP_000526135.1 IS200/IS605-like element IS200C family transposase -
QUE57_RS11215 (1939594) 1939594..1939968 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
QUE57_RS11220 (1939994) 1939994..1940212 + 219 WP_001291435.1 HHA domain-containing protein Toxin
QUE57_RS11225 (1940384) 1940384..1940935 + 552 WP_000102564.1 maltose O-acetyltransferase -
QUE57_RS11230 (1941051) 1941051..1941521 + 471 WP_063085325.1 YlaC family protein -
QUE57_RS11235 (1941685) 1941685..1943235 + 1551 WP_063085326.1 cyclic-guanylate-specific phosphodiesterase PdeB -
QUE57_RS11240 (1943277) 1943277..1943630 - 354 WP_000878140.1 DUF1428 domain-containing protein -
QUE57_RS11250 (1944009) 1944009..1944320 + 312 WP_000409911.1 MGMT family protein -
QUE57_RS11255 (1944351) 1944351..1944923 - 573 WP_000779826.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 1938457..1938915 458


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T44057 WP_001291435.1 NZ_AP027436:1939994-1940212 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T44057 NZ_AP027436:1939994-1940212 [Escherichia coli]
ATGTCCGAAAAACCTTTAACAAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT44057 WP_000344800.1 NZ_AP027436:1939594-1939968 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT44057 NZ_AP027436:1939594-1939968 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References