Detailed information of TA system TA044056

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-HTH_XRE
• Location: 1489951..1490656
• Replicon: chromosome
• Accession: NZ_AP027436
• Organism: Escherichia coli strain EC571 isolate EC571

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A3A6SJV3
• Locus tag: QUE57_RS08990
• Protein ID: WP_063085620.1
• Coordinates: 1489951..1490337 (+)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: QUE57_RS08995
• Protein ID: WP_001280945.1
• Coordinates: 1490327..1490656 (+)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QUE57_RS08970 (1485955)	1485955..1486581	+	627	WP_001314584.1	glutathione S-transferase GstB	-
QUE57_RS08975 (1486578)	1486578..1487693	-	1116	WP_063085618.1	aldose sugar dehydrogenase YliI	-
QUE57_RS08980 (1487804)	1487804..1488187	-	384	WP_000497137.1	biofilm formation regulator BssR	-
QUE57_RS08985 (1488400)	1488400..1489725	+	1326	WP_000049367.1	30S ribosomal protein S12 methylthiotransferase RimO	-
QUE57_RS08990 (1489951)	1489951..1490337	+	387	WP_063085620.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
QUE57_RS08995 (1490327)	1490327..1490656	+	330	WP_001280945.1	DNA-binding transcriptional regulator	Antitoxin
QUE57_RS09000 (1490726)	1490726..1492054	-	1329	WP_000086873.1	GGDEF domain-containing protein	-
QUE57_RS09005 (1492062)	1492062..1494410	-	2349	WP_021523051.1	EAL domain-containing protein	-
QUE57_RS09010 (1494588)	1494588..1495499	-	912	WP_001236019.1	glutathione ABC transporter permease GsiD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-124)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-94)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14280.45 Da
• Isoelectric Point: 9.9296

>T44056 WP_063085620.1 NZ_AP027436:1489951-1490337 [Escherichia coli]
MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVIKKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSG
LSSKGTKEIEDDELAAYKKMANAFLAFSTKQIEDLIETGFLIEVKNER

Nucleotide

• Length: 387 bp

>T44056 NZ_AP027436:1489951-1490337 [Escherichia coli]
ATGGGTGTGTATAAGGCAAGACGATTCAGCCAAAGCACGAAAAAACTTGGTATTCACGATAAAGTTCTCATGGCTGCCGC
TGAAGAAGTGATGCAGGGCATATGGGAGGCTGATTTAGGTAGCGGTGTCATCAAAAAACGTTTACCCCTCCAACAAGGAA
AGAGTGGTGGAGCCAGAACAATTATCTTTTTTAAATCAGCAAATCATGTGTTTTTTTATGATGGTTGGTCCAAGAGTGGT
CTAAGCAGTAAAGGAACCAAAGAAATCGAAGATGATGAACTGGCGGCCTACAAAAAAATGGCAAATGCATTTCTGGCCTT
TAGCACTAAGCAAATAGAGGATTTGATCGAAACTGGCTTTTTGATTGAGGTGAAAAATGAGCGATAA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11982.08 Da
• Isoelectric Point: 10.0101

>AT44056 WP_001280945.1 NZ_AP027436:1490327-1490656 [Escherichia coli]
MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAKVKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSK
WERNEIKPSGPALRILNTLAIKGPEVFAK

Nucleotide

• Length: 330 bp

>AT44056 NZ_AP027436:1490327-1490656 [Escherichia coli]
ATGAGCGATAAATTACAAATGATGCTGGAAGATGCAAAGGAACTGAATGCACTGGGCCTGATGTCTGATAGTGAAGTTGA
TGCCATTGCCGTCATGGTCAAAGCTCGTGAAGTCAGCGCCCGTATAGCCAAAGTCAAATCAATGACGGGTGAAGAGATCA
AAGCCATCCGCACCCGATACGGCATGTCACAATCCGTGCTGGCACATACAATGGGAATGTCAAAAGAAAGTGTCTCGAAA
TGGGAACGTAATGAAATAAAACCGAGCGGCCCGGCATTACGTATTTTGAATACATTGGCAATAAAAGGGCCGGAAGTTTT
TGCAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A3A6SJV3