Detailed information of TA system TA044054

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 779008..779646
• Replicon: chromosome
• Accession: NZ_AP027436
• Organism: Escherichia coli strain EC571 isolate EC571

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A3A6S5Q3
• Locus tag: QUE57_RS05420
• Protein ID: WP_063085535.1
• Coordinates: 779470..779646 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: QUE57_RS05415
• Protein ID: WP_001270286.1
• Coordinates: 779008..779424 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QUE57_RS05395 (774160)	774160..775101	-	942	WP_063085538.1	ABC transporter permease	-
QUE57_RS05400 (775102)	775102..776115	-	1014	WP_063085537.1	ABC transporter ATP-binding protein	-
QUE57_RS05405 (776133)	776133..777278	-	1146	WP_000047450.1	ABC transporter substrate-binding protein	-
QUE57_RS05410 (777523)	777523..778929	-	1407	WP_063085536.1	PLP-dependent aminotransferase family protein	-
QUE57_RS05415 (779008)	779008..779424	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
QUE57_RS05420 (779470)	779470..779646	-	177	WP_063085535.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
QUE57_RS05425 (779868)	779868..780098	+	231	WP_000494244.1	YncJ family protein	-
QUE57_RS05430 (780190)	780190..782151	-	1962	WP_063085534.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
QUE57_RS05435 (782224)	782224..782760	-	537	WP_000429507.1	DNA-binding transcriptional regulator SutR	-
QUE57_RS05440 (782852)	782852..784023	+	1172	Protein_810	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6751.78 Da
• Isoelectric Point: 11.5666

>T44054 WP_063085535.1 NZ_AP027436:c779646-779470 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFQGRRSVMPRHPNDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T44054 NZ_AP027436:c779646-779470 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTGGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCAAGGGCGACGCAGTGTCATGCCGCGTCACCCCAATGATGAGATAAAAGAACCATTGCGTAAAGCAATCCTGAAGC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT44054 WP_001270286.1 NZ_AP027436:c779424-779008 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT44054 NZ_AP027436:c779424-779008 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTGCCTTTGAGCGTCGCCTCTAAAGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAAAT
TACTCGCTTATTTAACTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTTGCGGCTAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• AlphaFold DB: A0A3A6S5Q3