Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/RHH(antitoxin)
Location 3755..4314 Replicon plasmid pEC571_2
Accession NZ_AP027434
Organism Escherichia coli strain EC571 isolate EC571

Toxin (Protein)


Gene name relE Uniprot ID S1PMX5
Locus tag QUE57_RS00465 Protein ID WP_000421257.1
Coordinates 3755..4030 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID C1J8K5
Locus tag QUE57_RS00470 Protein ID WP_001178089.1
Coordinates 4030..4314 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QUE57_RS00445 (MUTS10_00780) 91..456 + 366 WP_000091308.1 hypothetical protein -
QUE57_RS00450 (MUTS10_00790) 456..1643 + 1188 WP_000937591.1 IS91 family transposase -
QUE57_RS00455 (MUTS10_00800) 1919..2284 + 366 WP_000124096.1 hypothetical protein -
QUE57_RS00460 (MUTS10_00810) 2284..3471 + 1188 WP_286338237.1 IS91-like element IS91 family transposase -
QUE57_RS00465 (MUTS10_00820) 3755..4030 - 276 WP_000421257.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
QUE57_RS00470 (MUTS10_00830) 4030..4314 - 285 WP_001178089.1 ribbon-helix-helix domain-containing protein Antitoxin
QUE57_RS00475 (MUTS10_00840) 5226..6083 - 858 WP_000130988.1 incFII family plasmid replication initiator RepA -
QUE57_RS00480 6076..6150 - 75 WP_001365705.1 RepA leader peptide Tap -
QUE57_RS00485 (MUTS10_00850) 6389..6643 - 255 WP_000083830.1 replication regulatory protein RepA -
QUE57_RS00490 (MUTS10_00860) 6883..7473 - 591 WP_000766807.1 DUF2726 domain-containing protein -
QUE57_RS00495 (MUTS10_00870) 7511..7720 - 210 WP_001610317.1 hemolysin expression modulator Hha -
QUE57_RS00500 (MUTS10_00880) 7766..8227 - 462 WP_001233868.1 thermonuclease family protein -
QUE57_RS00505 8426..8677 - 252 WP_001372275.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid mcr-5.1 hlyC / hlyA / hlyB / hlyD 1..72586 72586


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(3-90)

Antitoxin

Ribbon-helix-helix protein, CopG

(14-51)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10749.45 Da        Isoelectric Point: 9.9324

>T44037 WP_000421257.1 NZ_AP027434:c4030-3755 [Escherichia coli]
MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIY
VLRLWHTRENR

Download         Length: 276 bp

>T44037 NZ_AP027434:c4030-3755 [Escherichia coli]
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTGGCCAGTAAACCTGC
TGCCGCCAGAACGGTACAGTCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAACTTAATGGCCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10456.86 Da        Isoelectric Point: 5.6558

>AT44037 WP_001178089.1 NZ_AP027434:c4314-4030 [Escherichia coli]
IQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDNPLPVPR

Download         Length: 285 bp

>AT44037 NZ_AP027434:c4314-4030 [Escherichia coli]
ATACAAATGAAAAACAATACCGCACAGGCAACAAAAGTCATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGGCTGGAACGCTCCCGGGGCTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAAGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATCGACCATCAGGCTGTACAGGCCTGG
GCGGACAGTCTCAGTACTGACAATCCGTTACCGGTGCCCCGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PMX5

Antitoxin

Source ID Structure
AlphaFold DB A0A731IHA8

References