Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4486500..4487118 Replicon chromosome
Accession NZ_AP027432
Organism Escherichia coli strain EC581 isolate EC581

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag QUE97_RS24395 Protein ID WP_001291435.1
Coordinates 4486500..4486718 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag QUE97_RS24400 Protein ID WP_000344800.1
Coordinates 4486744..4487118 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QUE97_RS24360 (4481789) 4481789..4482361 + 573 WP_000779826.1 YbaY family lipoprotein -
QUE97_RS24365 (4482392) 4482392..4482703 - 312 WP_000409911.1 MGMT family protein -
QUE97_RS24375 (4483082) 4483082..4483435 + 354 WP_000878140.1 DUF1428 domain-containing protein -
QUE97_RS24380 (4483477) 4483477..4485027 - 1551 WP_063085326.1 cyclic-guanylate-specific phosphodiesterase PdeB -
QUE97_RS24385 (4485191) 4485191..4485661 - 471 WP_063085325.1 YlaC family protein -
QUE97_RS24390 (4485777) 4485777..4486328 - 552 WP_000102564.1 maltose O-acetyltransferase -
QUE97_RS24395 (4486500) 4486500..4486718 - 219 WP_001291435.1 HHA domain-containing protein Toxin
QUE97_RS24400 (4486744) 4486744..4487118 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
QUE97_RS24405 (4487797) 4487797..4488255 + 459 WP_000526135.1 IS200/IS605-like element IS200C family transposase -
QUE97_RS24410 (4488375) 4488375..4491524 - 3150 WP_001132480.1 efflux RND transporter permease AcrB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 4487797..4488255 458


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T44035 WP_001291435.1 NZ_AP027432:c4486718-4486500 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T44035 NZ_AP027432:c4486718-4486500 [Escherichia coli]
ATGTCCGAAAAACCTTTAACAAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT44035 WP_000344800.1 NZ_AP027432:c4487118-4486744 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT44035 NZ_AP027432:c4487118-4486744 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References