Detailed information of TA system TA044034

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mosAT/AbiEii-AbiEi
• Location: 4279751..4281440
• Replicon: chromosome
• Accession: NZ_AP027432
• Organism: Escherichia coli strain EC581 isolate EC581

Toxin (Protein)

• Gene name: mosT
• Uniprot ID: A0A3A6SPN4
• Locus tag: QUE97_RS23440
• Protein ID: WP_063085362.1
• Coordinates: 4280520..4281440 (+)
• Length: 307 a.a.

Antitoxin (Protein)

• Gene name: mosA
• Uniprot ID: -
• Locus tag: QUE97_RS23435
• Protein ID: WP_063502601.1
• Coordinates: 4279751..4280533 (+)
• Length: 261 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QUE97_RS23430 (4276369)	4276369..4279467	-	3099	WP_286338344.1	type I restriction endonuclease	-
QUE97_RS23435 (4279751)	4279751..4280533	+	783	WP_063502601.1	type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein	Antitoxin
QUE97_RS23440 (4280520)	4280520..4281440	+	921	WP_063085362.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
QUE97_RS23445 (4281874)	4281874..4282758	-	885	WP_001119037.1	LysR family transcriptional regulator	-
QUE97_RS23450 (4283023)	4283023..4284081	+	1059	WP_122987789.1	alpha/beta hydrolase	-
QUE97_RS23455 (4284223)	4284223..4285350	+	1128	WP_042028035.1	MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (14-261)

Antitoxin

• Transcriptional regulator AbiEi antitoxin, N-terminal domain (3-90)

Sequences

Toxin

Protein

• Length: 307 a.a.
• Molecular weight: 34587.06 Da
• Isoelectric Point: 7.3238

>T44034 WP_063085362.1 NZ_AP027432:4280520-4281440 [Escherichia coli]
MDLRDSYARQVKLLMAALPHVAKESCFALKGGTAINLFVQDFPRLSVDIDLAYKSFADRDTDLAAINDALKRITASLNGR
PGITAIRQENKADEKRIIVNTADAKIKIEVSPVWRGLLLPPAEMPVCERVEMEYGFTTMSVVSLADLYGGKICAALDRQH
PRDLFDVLNMLEKPGLMREIFDGFLCYLAGHPRPIAELLAPNWDIERITTLYAQEFSGMTQQETSLESLLSVTTLLPQAL
KSHLTDLDREFLLSFKQNSPDWSRYRYPEIQHLPAIRWKQRNLAVLKDKNPAKYVAAVNKLERVLA

Nucleotide

• Length: 921 bp

>T44034 NZ_AP027432:4280520-4281440 [Escherichia coli]
ATGGATCTGCGTGATAGTTATGCCCGGCAGGTTAAGCTGTTGATGGCTGCTCTGCCGCATGTGGCGAAGGAGTCCTGTTT
CGCGTTGAAAGGCGGCACGGCAATCAATTTGTTTGTGCAGGATTTTCCCCGTCTGTCGGTGGATATTGATCTGGCGTATA
AGAGCTTTGCGGATCGCGATACTGATCTGGCGGCTATCAACGACGCTTTGAAGAGGATTACTGCATCGCTGAACGGCAGA
CCGGGGATCACCGCGATTCGGCAGGAAAATAAAGCGGATGAAAAGCGCATCATTGTGAATACCGCTGATGCAAAGATTAA
GATCGAGGTTAGCCCGGTCTGGCGCGGGCTGTTGTTGCCTCCGGCAGAAATGCCTGTGTGCGAACGTGTGGAAATGGAGT
ATGGCTTCACCACCATGAGTGTCGTCTCGCTGGCGGATCTTTATGGCGGTAAAATCTGCGCAGCGCTTGATCGTCAGCAC
CCGCGCGATCTGTTTGATGTGCTGAATATGCTGGAGAAACCGGGACTGATGCGGGAGATCTTTGACGGTTTTCTTTGTTA
TCTGGCAGGCCATCCCCGCCCCATTGCCGAGTTGCTGGCCCCAAACTGGGATATAGAGCGGATCACGACGCTGTATGCTC
AGGAATTTTCGGGGATGACTCAGCAGGAAACGTCGCTGGAATCGCTGCTGTCAGTAACGACTTTGCTGCCGCAGGCGTTG
AAGTCACATCTTACGGATCTGGATCGCGAGTTCTTGCTCAGTTTTAAGCAAAACAGCCCTGACTGGTCGCGGTATCGCTA
TCCTGAAATTCAGCATCTTCCGGCTATTCGCTGGAAGCAACGTAATCTGGCGGTGCTGAAAGATAAAAATCCGGCGAAGT
ATGTGGCGGCGGTGAATAAGCTAGAGAGGGTGCTGGCGTAA

Antitoxin

Protein

• Length: 261 a.a.
• Molecular weight: 29094.32 Da
• Isoelectric Point: 8.1223

>AT44034 WP_063502601.1 NZ_AP027432:4279751-4280533 [Escherichia coli]
MPNYLNWLMQNTLPGQVLLQSWLTRNGVDRKLSYLYVQNGWLRRIAHGVYCRTGREPDWVDAVYCLQTQWEKPVRVAGLT
SLALQGHAHYTEPGRPQVWLALPAYVKLPGWFAAFEQSATFITLYTSGLTVDVTSLTTELKIGDCQLAGSVPELAAYEIA
CGVPSLISFEHADALFQGLNLLSPRKLQALLRASHSVKTNRVMLYLARRNGHPYFQYIDQSGIETGTGKRQIIPGGWLEP
DYQITVPRAFKPEGVEDGSA

Nucleotide

• Length: 783 bp

>AT44034 NZ_AP027432:4279751-4280533 [Escherichia coli]
TTGCCAAACTATCTAAACTGGTTGATGCAAAATACGCTACCCGGTCAGGTGCTGCTCCAGTCATGGCTTACCCGCAACGG
CGTAGATCGTAAGCTTTCTTATCTCTATGTTCAAAATGGCTGGCTCAGGCGCATCGCTCACGGCGTGTACTGCCGGACGG
GCCGTGAGCCGGATTGGGTAGACGCGGTTTACTGCCTGCAAACACAGTGGGAAAAGCCTGTTCGGGTGGCGGGGCTAACC
AGCCTTGCGCTACAGGGCCATGCGCACTATACCGAACCGGGCAGGCCGCAGGTCTGGCTCGCGCTTCCGGCTTACGTTAA
GCTGCCGGGATGGTTTGCTGCCTTCGAACAATCTGCAACATTTATCACGCTGTATACATCGGGGCTTACCGTAGATGTAA
CGTCATTGACTACGGAACTAAAAATTGGCGACTGCCAGTTAGCAGGCAGTGTTCCGGAACTGGCCGCGTATGAGATCGCC
TGCGGCGTTCCTTCGTTGATCTCGTTCGAACATGCCGATGCTCTGTTTCAGGGGTTGAATCTTCTCAGCCCACGCAAATT
GCAGGCACTGCTTCGCGCCAGTCATTCGGTTAAAACCAACCGGGTGATGCTTTACCTTGCCCGGCGTAATGGACACCCTT
ATTTTCAGTATATTGATCAAAGCGGTATAGAAACAGGTACGGGAAAGCGGCAAATCATACCCGGCGGCTGGCTGGAGCCG
GATTATCAAATTACTGTTCCACGCGCATTTAAACCGGAAGGAGTCGAAGATGGATCTGCGTGA

Structures

Toxin

• AlphaFold DB: A0A3A6SPN4