Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 3928083..3928659 Replicon chromosome
Accession NZ_AP027432
Organism Escherichia coli strain EC581 isolate EC581

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag QUE97_RS21745 Protein ID WP_001297643.1
Coordinates 3928083..3928370 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A080I7H7
Locus tag QUE97_RS21750 Protein ID WP_000063160.1
Coordinates 3928357..3928659 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QUE97_RS21725 (3924336) 3924336..3925616 - 1281 WP_001037400.1 DUF445 domain-containing protein -
QUE97_RS21730 (3925657) 3925657..3926889 - 1233 WP_024186283.1 multidrug efflux MFS transporter MdtM -
QUE97_RS21735 (3927035) 3927035..3927130 - 96 Protein_3771 dienelactone hydrolase family protein -
QUE97_RS21740 (3927187) 3927187..3927884 + 698 WP_252702861.1 IS1 family transposase -
QUE97_RS21745 (3928083) 3928083..3928370 + 288 WP_001297643.1 BrnT family toxin Toxin
QUE97_RS21750 (3928357) 3928357..3928659 + 303 WP_000063160.1 BrnA antitoxin family protein Antitoxin
QUE97_RS21755 (3928693) 3928693..3929649 - 957 WP_001297640.1 GTPase -
QUE97_RS21760 (3929660) 3929660..3929863 - 204 WP_000467859.1 YbdD/YjiX family protein -
QUE97_RS21765 (3929913) 3929913..3932063 - 2151 WP_001295524.1 pyruvate/proton symporter BtsT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 3927591..3927884 293


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T44032 WP_001297643.1 NZ_AP027432:3928083-3928370 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T44032 NZ_AP027432:3928083-3928370 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGAAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTATTGTTTGATGATCCACAACATTTGTCACAGCAAGAACGCATAGAAAACGGTGAGTATCGCTGGCAGACAATCGGCC
TGGTTTACGGCATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAATCGCTATGAGCATGGTTAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11552.01 Da        Isoelectric Point: 9.4646

>AT44032 WP_000063160.1 NZ_AP027432:3928357-3928659 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALTNKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT44032 NZ_AP027432:3928357-3928659 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCGCTGACGAA
TAAATCCGACGAAGATATTGATTACAGTGATATCCCACCTTCCAGCGATGAGCAATGGTCTAACGCCGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGTTGAAGCGACCAGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A080I7H7

References