Detailed information of TA system TA044013

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 599231..599869
• Replicon: chromosome
• Accession: NZ_AP027432
• Organism: Escherichia coli strain EC581 isolate EC581

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A3A6S5Q3
• Locus tag: QUE97_RS05405
• Protein ID: WP_063085535.1
• Coordinates: 599231..599407 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: QUE97_RS05410
• Protein ID: WP_001270286.1
• Coordinates: 599453..599869 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QUE97_RS05385 (594854)	594854..596025	-	1172	Protein_585	BenE family transporter YdcO	-
QUE97_RS05390 (596117)	596117..596653	+	537	WP_000429507.1	DNA-binding transcriptional regulator SutR	-
QUE97_RS05395 (596726)	596726..598687	+	1962	WP_063085534.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
QUE97_RS05400 (598779)	598779..599009	-	231	WP_000494244.1	YncJ family protein	-
QUE97_RS05405 (599231)	599231..599407	+	177	WP_063085535.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
QUE97_RS05410 (599453)	599453..599869	+	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
QUE97_RS05415 (599948)	599948..601354	+	1407	WP_063085536.1	PLP-dependent aminotransferase family protein	-
QUE97_RS05420 (601599)	601599..602744	+	1146	WP_000047450.1	ABC transporter substrate-binding protein	-
QUE97_RS05425 (602762)	602762..603775	+	1014	WP_063085537.1	ABC transporter ATP-binding protein	-
QUE97_RS05430 (603776)	603776..604717	+	942	WP_063085538.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6751.78 Da
• Isoelectric Point: 11.5666

>T44013 WP_063085535.1 NZ_AP027432:599231-599407 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFQGRRSVMPRHPNDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T44013 NZ_AP027432:599231-599407 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTGGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCAAGGGCGACGCAGTGTCATGCCGCGTCACCCCAATGATGAGATAAAAGAACCATTGCGTAAAGCAATCCTGAAGC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT44013 WP_001270286.1 NZ_AP027432:599453-599869 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT44013 NZ_AP027432:599453-599869 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTGCCTTTGAGCGTCGCCTCTAAAGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAAAT
TACTCGCTTATTTAACTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTTGCGGCTAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• AlphaFold DB: A0A3A6S5Q3