Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 206821..207655 Replicon chromosome
Accession NZ_AP027432
Organism Escherichia coli strain EC581 isolate EC581

Toxin (Protein)


Gene name yeeV Uniprot ID A0A3S7R4W2
Locus tag QUE97_RS03455 Protein ID WP_063085447.1
Coordinates 207278..207655 (+) Length 126 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A2J4HRK6
Locus tag QUE97_RS03450 Protein ID WP_001354276.1
Coordinates 206821..207189 (+) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QUE97_RS03430 (202239) 202239..203111 + 873 WP_053271975.1 GTPase family protein -
QUE97_RS03435 (203484) 203484..205694 + 2211 Protein_200 Ag43/Cah family autotransporter adhesin -
QUE97_RS03440 (205689) 205689..206159 + 471 Protein_201 DUF932 domain-containing protein -
QUE97_RS03445 (206250) 206250..206564 + 315 Protein_202 antirestriction protein -
QUE97_RS03450 (206821) 206821..207189 + 369 WP_001354276.1 type IV toxin-antitoxin system cytoskeleton bundling-enhancing antitoxin CbeA Antitoxin
QUE97_RS03455 (207278) 207278..207655 + 378 WP_063085447.1 type IV toxin-antitoxin system cytoskeleton-binding toxin CbtA Toxin
QUE97_RS03460 (207652) 207652..208140 + 489 WP_068862750.1 DUF5983 family protein -
QUE97_RS03465 (208152) 208152..208349 + 198 WP_029396577.1 DUF957 domain-containing protein -
QUE97_RS03470 (208434) 208434..209276 + 843 WP_236272896.1 DUF4942 domain-containing protein -
QUE97_RS03480 (209747) 209747..210685 + 939 WP_001357083.1 glyoxylate/hydroxypyruvate reductase GhrA -
QUE97_RS03485 (210740) 210740..211477 + 738 WP_000283664.1 zinc-binding phosphatase -
QUE97_RS03490 (211501) 211501..212055 + 555 WP_063085449.1 molecular chaperone YcdY -
QUE97_RS03495 (212157) 212157..212648 + 492 WP_032140678.1 DUF1097 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 13908.94 Da        Isoelectric Point: 8.5222

>T44012 WP_063085447.1 NZ_AP027432:207278-207655 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNNTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTHSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPGAKQ

Download         Length: 378 bp

>T44012 NZ_AP027432:207278-207655 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTACGGCCTCACACTGAATAACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCACTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCACTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCCCGCCGGGCGACAGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAACATCCGGGGGCGAAACAATGA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13649.47 Da        Isoelectric Point: 7.0261

>AT44012 WP_001354276.1 NZ_AP027432:206821-207189 [Escherichia coli]
VSDTLSGTTHPDDNHDRPWWGLPCTVTPCFGARLVQEGNQLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYARGLTCKADTLSSCGYVYLAVYPTPKMKN

Download         Length: 369 bp

>AT44012 NZ_AP027432:206821-207189 [Escherichia coli]
GTGTCAGACACACTCTCCGGGACAACGCATCCCGATGACAATCATGACCGTCCCTGGTGGGGACTACCCTGCACCGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGCAATCAGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATGCGTACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAGCTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCCAGAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCAAAATGAAAAATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3S7R4W2

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4HRK6

References