Detailed information of TA system TA044007

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 1983..2542
• Replicon: plasmid pEC581_2
• Accession: NZ_AP027430
• Organism: Escherichia coli strain EC581 isolate EC581

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1PMX5
• Locus tag: QUE97_RS00850
• Protein ID: WP_000421257.1
• Coordinates: 2267..2542 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: QUE97_RS00845
• Protein ID: WP_273949219.1
• Coordinates: 1983..2267 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QUE97_RS00840 (46)	46..1077	+	1032	WP_000907869.1	plasmid replication initiator RepA	-
QUE97_RS00845 (1983)	1983..2267	+	285	WP_273949219.1	ribbon-helix-helix domain-containing protein	Antitoxin
QUE97_RS00850 (2267)	2267..2542	+	276	WP_000421257.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
QUE97_RS00855 (2648)	2648..2851	+	204	Protein_3	hypothetical protein	-
QUE97_RS00860 (3121)	3121..3387	-	267	WP_021576094.1	hypothetical protein	-
QUE97_RS00865 (3387)	3387..3881	-	495	WP_001459505.1	hypothetical protein	-
QUE97_RS00870 (3937)	3937..4539	-	603	WP_097468803.1	ProQ/FINO family protein	-
QUE97_RS00875 (4715)	4715..5767	-	1053	WP_000655519.1	hypothetical protein	-
QUE97_RS00880 (5980)	5980..6570	-	591	WP_053264278.1	hypothetical protein	-
QUE97_RS00885 (6570)	6570..6824	-	255	WP_000343103.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..83702	83702

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (14-51)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10749.45 Da
• Isoelectric Point: 9.9324

>T44007 WP_000421257.1 NZ_AP027430:2267-2542 [Escherichia coli]
MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIY
VLRLWHTRENR

Nucleotide

• Length: 276 bp

>T44007 NZ_AP027430:2267-2542 [Escherichia coli]
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTGGCCAGTAAACCTGC
GGCCGCCAGAACGGTACAGTCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAACTTAATGGCCAGACTATTTAT
GTATTACGCCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10474.89 Da
• Isoelectric Point: 5.6558

>AT44007 WP_273949219.1 NZ_AP027430:1983-2267 [Escherichia coli]
MQMKNNSAQATKVITAHVPLPMADKVDQMAARLERSRGWIIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDNPLPVPR

Nucleotide

• Length: 285 bp

>AT44007 NZ_AP027430:1983-2267 [Escherichia coli]
ATGCAAATGAAAAACAATAGTGCACAAGCAACAAAAGTCATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGCTCCCGGGGCTGGATTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATCGACCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Structures

Toxin

• AlphaFold DB: S1PMX5