Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-YafN
Location 68259..68794 Replicon plasmid pB27_2
Accession NZ_AP027426
Organism Escherichia coli strain B27 isolate B27

Toxin (Protein)


Gene name relE Uniprot ID A0A0K4CX11
Locus tag QUE78_RS24230 Protein ID WP_000222771.1
Coordinates 68507..68794 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID T1PXV2
Locus tag QUE78_RS24225 Protein ID WP_001139206.1
Coordinates 68259..68510 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QUE78_RS24220 (MUTS8_46800) 63709..67677 - 3969 Protein_74 helicase-related protein -
QUE78_RS24225 (MUTS8_46810) 68259..68510 + 252 WP_001139206.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
QUE78_RS24230 (MUTS8_46820) 68507..68794 + 288 WP_000222771.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
QUE78_RS24240 69940..70053 - 114 Protein_78 alkaline phosphatase -
QUE78_RS24245 70052..70257 + 206 Protein_79 hypothetical protein -
QUE78_RS24255 (MUTS8_46870) 72105..72350 - 246 Protein_81 IS91 family transposase -
QUE78_RS24260 (MUTS8_46880) 72412..72852 - 441 Protein_82 IS3 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid tet(A) / dfrA16 / blaCARB-2 / aadA2 / cmlA1 / ant(3'')-Ia / sul3 - 1..93860 93860


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-83)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11116.17 Da        Isoelectric Point: 10.5833

>T44005 WP_000222771.1 NZ_AP027426:68507-68794 [Escherichia coli]
MTYTVKFRDDALKEWLRLDKTIQQQFAKKLKKCSENPHIASAKLRGLKDCYKIKLRASGFRLVYQVIDDMLIIAVVAVGK
RERSNVYHLASERMK

Download         Length: 288 bp

>T44005 NZ_AP027426:68507-68794 [Escherichia coli]
ATGACTTATACAGTAAAATTCCGGGATGATGCACTGAAAGAATGGCTCAGACTGGACAAGACCATTCAGCAGCAGTTCGC
AAAAAAACTGAAAAAATGCAGTGAAAATCCTCATATAGCTTCTGCAAAACTACGTGGATTAAAGGACTGCTATAAAATCA
AACTTCGCGCATCAGGTTTTCGTCTGGTTTATCAGGTTATTGATGATATGTTAATTATTGCGGTCGTCGCAGTAGGTAAA
CGTGAACGCAGCAACGTTTATCATCTCGCCAGTGAACGGATGAAATAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9171.39 Da        Isoelectric Point: 4.0927

>AT44005 WP_001139206.1 NZ_AP027426:68259-68510 [Escherichia coli]
MPSIILSDTSASVSELKKNPMATVSAGEGYPVAILNRNQPAFYCVPAELYERMLDALDDQELVKLVTERSSQPLHDVDLD
SYL

Download         Length: 252 bp

>AT44005 NZ_AP027426:68259-68510 [Escherichia coli]
ATGCCCAGTATCATTTTGTCTGACACCAGTGCAAGCGTCAGTGAGCTGAAAAAAAATCCGATGGCAACTGTCAGTGCCGG
AGAGGGGTATCCTGTTGCCATTCTTAACCGGAATCAGCCAGCATTCTACTGTGTCCCTGCTGAGCTCTATGAAAGAATGC
TTGATGCCTTGGATGACCAGGAGCTGGTAAAACTGGTTACTGAACGCAGCAGTCAGCCTCTGCATGATGTGGACCTGGAC
AGTTATTTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0K4CX11

Antitoxin

Source ID Structure
AlphaFold DB T1PXV2

References