Detailed information of TA system TA044004

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-Phd
• Location: 31921..32522
• Replicon: plasmid pB27_2
• Accession: NZ_AP027426
• Organism: Escherichia coli strain B27 isolate B27

Toxin (Protein)

• Gene name: doc
• Uniprot ID: -
• Locus tag: QUE78_RS24005
• Protein ID: WP_001216039.1
• Coordinates: 32142..32522 (+)
• Length: 127 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: -
• Locus tag: QUE78_RS24000
• Protein ID: WP_001190711.1
• Coordinates: 31921..32142 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QUE78_RS23970 (MUTS8_46270)	27019..27717	+	699	Protein_24	IS1 family transposase	-
QUE78_RS23975 (MUTS8_46280)	27733..28281	+	549	Protein_25	metallophosphoesterase	-
QUE78_RS23980 (MUTS8_46290)	28278..28979	+	702	WP_000684873.1	hypothetical protein	-
QUE78_RS23985 (MUTS8_46310)	29281..30543	+	1263	WP_069067570.1	hypothetical protein	-
QUE78_RS23990 (MUTS8_46320)	30616..30942	+	327	Protein_28	hypothetical protein	-
QUE78_RS24000 (MUTS8_46350)	31921..32142	+	222	WP_001190711.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
QUE78_RS24005 (MUTS8_46360)	32142..32522	+	381	WP_001216039.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
QUE78_RS24010 (MUTS8_46370)	32527..32706	+	180	WP_000113018.1	hypothetical protein	-
QUE78_RS24015 (MUTS8_46380)	32734..33777	+	1044	WP_069067567.1	DUF968 domain-containing protein	-
QUE78_RS24020 (MUTS8_46400)	33993..34391	+	399	WP_229436862.1	phage antirepressor KilAC domain-containing protein	-
QUE78_RS24025 (MUTS8_46410)	34424..35275	-	852	WP_000611661.1	phage repressor protein C1	-
QUE78_RS24030 (MUTS8_46420)	35386..35595	-	210	WP_000874156.1	hypothetical protein	-
QUE78_RS24035 (MUTS8_46430)	36200..36421	+	222	WP_000542338.1	hypothetical protein	-
QUE78_RS24040 (MUTS8_46440)	36429..37460	+	1032	WP_000067537.1	site-specific integrase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	tet(A) / dfrA16 / blaCARB-2 / aadA2 / cmlA1 / ant(3'')-Ia / sul3	-	1..93860	93860

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (2-123)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-51)

Sequences

Toxin

Protein

• Length: 127 a.a.
• Molecular weight: 13558.27 Da
• Isoelectric Point: 5.1514

>T44004 WP_001216039.1 NZ_AP027426:32142-32522 [Escherichia coli]
MRHISPEELIALHDANISRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRAALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Nucleotide

• Length: 381 bp

>T44004 NZ_AP027426:32142-32522 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTGATAGCGCTTCATGATGCGAATATAAGCCGCTACGGCGGCCTGCCTGGCATGTC
AGATCCGGGCAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTGCCGCGCTAAACAGTGCG
CTGTTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACCGTAGGGGCTGCGACCGG
AGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCCGCGGAGTAG

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8134.08 Da
• Isoelectric Point: 4.5843

>AT44004 WP_001190711.1 NZ_AP027426:31921-32142 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVDR

Nucleotide

• Length: 222 bp

>AT44004 NZ_AP027426:31921-32142 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGCGGCAACCTTTCTGAAGTGCTCAACAATGTTGAGGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCTCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTGACCGATAA