Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-Phd
Location 127463..128064 Replicon plasmid pB27_1
Accession NZ_AP027425
Organism Escherichia coli strain B27 isolate B27

Toxin (Protein)


Gene name doc Uniprot ID V0AJ64
Locus tag QUE78_RS23805 Protein ID WP_001216034.1
Coordinates 127684..128064 (+) Length 127 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID U9YQH9
Locus tag QUE78_RS23800 Protein ID WP_001190712.1
Coordinates 127463..127684 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QUE78_RS23790 (124444) 124444..125727 - 1284 WP_001617890.1 restriction endonuclease subunit S -
QUE78_RS23795 (125724) 125724..127280 - 1557 WP_001617892.1 type I restriction-modification system subunit M -
QUE78_RS23800 (127463) 127463..127684 + 222 WP_001190712.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
QUE78_RS23805 (127684) 127684..128064 + 381 WP_001216034.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
QUE78_RS23810 (128069) 128069..128248 + 180 WP_001513661.1 hypothetical protein -
QUE78_RS23815 (128276) 128276..128554 + 279 Protein_148 pdcB -
QUE78_RS23820 (128559) 128559..128972 + 414 Protein_149 integrase core domain-containing protein -
QUE78_RS23825 (128922) 128922..129257 - 336 WP_169329198.1 type I deoxyribonuclease HsdR -
QUE78_RS23830 (129467) 129467..130447 - 981 WP_001567628.1 IS5-like element IS5 family transposase -
QUE78_RS23835 (130691) 130691..132094 + 1404 WP_001373486.1 S-methylmethionine permease -
QUE78_RS23840 (132081) 132081..133013 + 933 WP_000081352.1 homocysteine S-methyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaCMY-2 / aac(3)-IId / mcr-3.1 / mph(A) - 1..133820 133820


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(2-123)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-51)


Sequences

Toxin        


Download         Length: 127 a.a.        Molecular weight: 13615.32 Da        Isoelectric Point: 5.1514

>T44003 WP_001216034.1 NZ_AP027425:127684-128064 [Escherichia coli]
MRHISPEELIALHDANINRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Download         Length: 381 bp

>T44003 NZ_AP027425:127684-128064 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAACCGCTACGGCGGCCTGCCGGGAATGTC
TGATCCGGGCAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGTTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACCGTAGGGGCTGCGACCGG
AGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCTGCGGAGTAG

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8133.10 Da        Isoelectric Point: 4.7708

>AT44003 WP_001190712.1 NZ_AP027425:127463-127684 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Download         Length: 222 bp

>AT44003 NZ_AP027425:127463-127684 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGCGGCAACCTTTCTGAAGTGCTCAACAATGTTGAGGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0AJ64

Antitoxin

Source ID Structure
AlphaFold DB A0A829CJB6

References