Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 114819..115462 Replicon plasmid pB27_1
Accession NZ_AP027425
Organism Escherichia coli strain B27 isolate B27

Toxin (Protein)


Gene name vagD Uniprot ID B1LRW4
Locus tag QUE78_RS23760 Protein ID WP_001044768.1
Coordinates 115046..115462 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D2WFK3
Locus tag QUE78_RS23755 Protein ID WP_001261287.1
Coordinates 114819..115049 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QUE78_RS23735 (109999) 109999..111132 - 1134 WP_000545983.1 DUF3800 domain-containing protein -
QUE78_RS23740 (111395) 111395..113032 - 1638 WP_223201785.1 hypothetical protein -
QUE78_RS23745 (113083) 113083..113913 - 831 WP_223201786.1 hypothetical protein -
QUE78_RS23750 (113965) 113965..114513 - 549 WP_039002808.1 hypothetical protein -
QUE78_RS23755 (114819) 114819..115049 + 231 WP_001261287.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
QUE78_RS23760 (115046) 115046..115462 + 417 WP_001044768.1 type II toxin-antitoxin system VapC family toxin Toxin
QUE78_RS23765 (115624) 115624..117762 - 2139 WP_039002805.1 AAA family ATPase -
QUE78_RS23770 (118227) 118227..119222 + 996 WP_000246636.1 hypothetical protein -
QUE78_RS23775 (119265) 119265..120158 + 894 WP_001553853.1 S-4TM family putative pore-forming effector -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaCMY-2 / aac(3)-IId / mcr-3.1 / mph(A) - 1..133820 133820
- flank IS/Tn - - 120295..120672 377


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15197.66 Da        Isoelectric Point: 7.7805

>T44002 WP_001044768.1 NZ_AP027425:115046-115462 [Escherichia coli]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDEFCARLDAILPWDRA
AVDATTKIKVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK

Download         Length: 417 bp

>T44002 NZ_AP027425:115046-115462 [Escherichia coli]
GTGAACAAGACTTATATGCTTGATACCTGTATCTGCTCGTTCATCATGCGTGAACAGCCAGAAGCCGTCCTGAAGCGTCT
GGAGCAGGCGGTGCTACGCGGCCACCGTATAGTGGTCTCGGCCATCACCTACTCTGAAATGCGCTTCGGGGCTACCGGCC
CAAAGGCCTCACCACGTCACGTCCAACTGGTCGATGAATTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCC
GCAGTAGACGCCACCACGAAGATTAAAGTGGCACTGCGGCTGGCCGGGACGCCGATCGGCCCGAACGACACGGCGATTGC
CGGGCACGCCATCGCCGCCGGGGCGATACTGGTAACGAATAATACAAGAGAATTTGAGCGAGTGCCTGACCTGGTGCTGG
AAGACTGGGTGAAGTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8815.90 Da        Isoelectric Point: 4.3775

>AT44002 WP_001261287.1 NZ_AP027425:114819-115049 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLEYEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT44002 NZ_AP027425:114819-115049 [Escherichia coli]
ATGAGAACCGTATCGATATTTAAAAATGGCAACAACCGCGCCATCCGTCTGCCTCGTGATCTGGATTTTGAGGGGGTGAG
CGAGCTCGAGATCGTCCGGGAAGGGGACAGCATCATCCTGCGCCCCGTCCGGCCAACCTGGGGATCATTCCTAGAGTACG
AAAAAGCCGATCCAGATTTTATGGCGGAGCGCGAGGATGTTGTCAGCGACGAAGGGCGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A606Q844

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QFC4

References