Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4023065..4023641 Replicon chromosome
Accession NZ_AP027424
Organism Escherichia coli strain B27 isolate B27

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag QUE78_RS19905 Protein ID WP_001297643.1
Coordinates 4023354..4023641 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A829G8Z0
Locus tag QUE78_RS19900 Protein ID WP_000063156.1
Coordinates 4023065..4023367 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QUE78_RS19885 (4019661) 4019661..4021811 + 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
QUE78_RS19890 (4021861) 4021861..4022064 + 204 WP_000467859.1 YbdD/YjiX family protein -
QUE78_RS19895 (4022075) 4022075..4023031 + 957 WP_001297640.1 GTPase -
QUE78_RS19900 (4023065) 4023065..4023367 - 303 WP_000063156.1 BrnA antitoxin family protein Antitoxin
QUE78_RS19905 (4023354) 4023354..4023641 - 288 WP_001297643.1 BrnT family toxin Toxin
QUE78_RS19910 (4023840) 4023840..4024537 - 698 Protein_3889 IS1 family transposase -
QUE78_RS19915 (4024594) 4024594..4024689 + 96 Protein_3890 dienelactone hydrolase family protein -
QUE78_RS19920 (4024835) 4024835..4026067 + 1233 WP_149543508.1 multidrug efflux MFS transporter MdtM -
QUE78_RS19925 (4026108) 4026108..4027388 + 1281 WP_001037400.1 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T43992 WP_001297643.1 NZ_AP027424:c4023641-4023354 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T43992 NZ_AP027424:c4023641-4023354 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGAAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTATTGTTTGATGATCCACAACATTTGTCACAGCAAGAACGTATAGAAAACGGTGAGTATCGCTGGCAGACAATCGGCC
TGGTTTACGGCATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAATCGCTATGAGCATGGTTAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11521.99 Da        Isoelectric Point: 9.4646

>AT43992 WP_000063156.1 NZ_AP027424:c4023367-4023065 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT43992 NZ_AP027424:c4023367-4023065 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCGCTGGCGAA
TAAATCCGACGAAGATATTGATTACAGTGATATCCCACCTTCCAGCGATGAGCAATGGTCTAACGCCGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGTTGAAGCGACCAGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A829G8Z0

References