Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 3914852..3915402 Replicon chromosome
Accession NZ_AP027424
Organism Escherichia coli strain B27 isolate B27

Toxin (Protein)


Gene name ccdB Uniprot ID D7Y6T6
Locus tag QUE78_RS19375 Protein ID WP_001160971.1
Coordinates 3914852..3915166 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID V0VEX8
Locus tag QUE78_RS19380 Protein ID WP_000125566.1
Coordinates 3915169..3915402 (-) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QUE78_RS19350 (3910527) 3910527..3911813 + 1287 WP_076486862.1 peptidylprolyl isomerase SurA -
QUE78_RS19355 (3911813) 3911813..3912802 + 990 WP_000241253.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
QUE78_RS19360 (3912799) 3912799..3913620 + 822 WP_001065381.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
QUE78_RS19365 (3913623) 3913623..3914000 + 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
QUE78_RS19370 (3914007) 3914007..3914855 + 849 WP_000257189.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
QUE78_RS19375 (3914852) 3914852..3915166 - 315 WP_001160971.1 CcdB family protein Toxin
QUE78_RS19380 (3915169) 3915169..3915402 - 234 WP_000125566.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
QUE78_RS19385 (3915488) 3915488..3915967 - 480 WP_001487721.1 type 3 dihydrofolate reductase -
QUE78_RS19390 (3916159) 3916159..3918021 - 1863 WP_072662688.1 glutathione-regulated potassium-efflux system protein KefC -
QUE78_RS19395 (3918014) 3918014..3918544 - 531 WP_000600735.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
QUE78_RS19400 (3918652) 3918652..3919984 - 1333 Protein_3790 MFS transporter -
QUE78_RS19405 (3920043) 3920043..3920330 - 288 WP_000203741.1 ferredoxin-like protein FixX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-103)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11741.75 Da        Isoelectric Point: 9.0851

>T43991 WP_001160971.1 NZ_AP027424:c3915166-3914852 [Escherichia coli]
MQFTVYRSRSRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Download         Length: 315 bp

>T43991 NZ_AP027424:c3915166-3914852 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCAGCAGGAACGCCGCGTTTCCCTTTGTTATCGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCCGTATTGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGGTAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8530.53 Da        Isoelectric Point: 5.8062

>AT43991 WP_000125566.1 NZ_AP027424:c3915402-3915169 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT43991 NZ_AP027424:c3915402-3915169 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A241QVQ9

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LTM9

References