Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 4219641..4220272 Replicon chromosome
Accession NZ_AP027423
Organism Escherichia coli strain EC261 isolate EC261

Toxin (Protein)


Gene name hicA Uniprot ID F4NNK6
Locus tag QUE58_RS23135 Protein ID WP_001259388.1
Coordinates 4219997..4220272 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag QUE58_RS23130 Protein ID WP_000593555.1
Coordinates 4219641..4220000 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QUE58_RS23105 (4215773) 4215773..4216717 + 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -
QUE58_RS23110 (4216714) 4216714..4217547 + 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
QUE58_RS23115 (4217547) 4217547..4218311 + 765 WP_001136236.1 nickel import ATP-binding protein NikD -
QUE58_RS23120 (4218308) 4218308..4219114 + 807 WP_000173666.1 nickel import ATP-binding protein NikE -
QUE58_RS23125 (4219120) 4219120..4219521 + 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
QUE58_RS23130 (4219641) 4219641..4220000 - 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
QUE58_RS23135 (4219997) 4219997..4220272 - 276 WP_001259388.1 type II toxin-antitoxin system HicA family toxin Toxin
QUE58_RS23140 (4220345) 4220345..4221469 - 1125 WP_001314210.1 ABC transporter permease -
QUE58_RS23145 (4221469) 4221469..4224204 - 2736 WP_000149125.1 ribosome-associated ATPase/putative transporter RbbA -
QUE58_RS23150 (4224201) 4224201..4225268 - 1068 WP_000361485.1 HlyD family secretion protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10151.89 Da        Isoelectric Point: 10.7076

>T43970 WP_001259388.1 NZ_AP027423:c4220272-4219997 [Escherichia coli]
MRTKVQALRKKQKNTLDQIVKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T43970 NZ_AP027423:c4220272-4219997 [Escherichia coli]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATAGTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGCAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT43970 WP_000593555.1 NZ_AP027423:c4220000-4219641 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT43970 NZ_AP027423:c4220000-4219641 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCAGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A0FFA2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References