Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 3508904..3509487 Replicon chromosome
Accession NZ_AP027423
Organism Escherichia coli strain EC261 isolate EC261

Toxin (Protein)


Gene name mazF Uniprot ID U9XFN8
Locus tag QUE58_RS19585 Protein ID WP_000254745.1
Coordinates 3508904..3509239 (-) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag QUE58_RS19590 Protein ID WP_000581937.1
Coordinates 3509239..3509487 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QUE58_RS19570 (3504791) 3504791..3506089 - 1299 WP_000036723.1 phosphopyruvate hydratase -
QUE58_RS19575 (3506177) 3506177..3507814 - 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
QUE58_RS19580 (3508042) 3508042..3508833 - 792 WP_001071648.1 nucleoside triphosphate pyrophosphohydrolase -
QUE58_RS19585 (3508904) 3508904..3509239 - 336 WP_000254745.1 endoribonuclease MazF Toxin
QUE58_RS19590 (3509239) 3509239..3509487 - 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
QUE58_RS19595 (3509565) 3509565..3511799 - 2235 WP_000226815.1 GTP pyrophosphokinase -
QUE58_RS19600 (3511847) 3511847..3513148 - 1302 WP_000046785.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12158.08 Da        Isoelectric Point: 8.4777

>T43967 WP_000254745.1 NZ_AP027423:c3509239-3508904 [Escherichia coli]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPYTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

>T43967 NZ_AP027423:c3509239-3508904 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTATACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT43967 WP_000581937.1 NZ_AP027423:c3509487-3509239 [Escherichia coli]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp

>AT43967 NZ_AP027423:c3509487-3509239 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYM3

Antitoxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CQY
PDB 1MVF
PDB 2MRN
PDB 2MRU
AlphaFold DB A0A7U9LMB4

References