Detailed information of TA system TA043943

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 1042860..1043697
• Replicon: chromosome
• Accession: NZ_AP027423
• Organism: Escherichia coli strain EC261 isolate EC261

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: Q3Z4X7
• Locus tag: QUE58_RS07450
• Protein ID: WP_000227784.1
• Coordinates: 1042860..1043402 (-)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: QUE58_RS07455
• Protein ID: WP_001297137.1
• Coordinates: 1043386..1043697 (-)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QUE58_RS07430 (1038399)	1038399..1039310	-	912	WP_000705874.1	2-dehydropantoate 2-reductase	-
QUE58_RS07435 (1039478)	1039478..1039969	+	492	WP_001138904.1	nucleotide binding protein YajQ	-
QUE58_RS07440 (1040097)	1040097..1041461	-	1365	WP_001000974.1	MFS transporter	-
QUE58_RS07445 (1041869)	1041869..1042804	+	936	WP_001365761.1	tetratricopeptide repeat protein	-
QUE58_RS07450 (1042860)	1042860..1043402	-	543	WP_000227784.1	GNAT family N-acetyltransferase	Toxin
QUE58_RS07455 (1043386)	1043386..1043697	-	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
QUE58_RS07460 (1043882)	1043882..1044772	-	891	WP_000971336.1	heme o synthase	-
QUE58_RS07465 (1044784)	1044784..1045113	-	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
QUE58_RS07470 (1045113)	1045113..1045727	-	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
QUE58_RS07475 (1045717)	1045717..1047708	-	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
QUE58_RS07480 (1047730)	1047730..1048677	-	948	WP_001239441.1	cytochrome o ubiquinol oxidase subunit II	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19805.02 Da
• Isoelectric Point: 8.3395

>T43943 WP_000227784.1 NZ_AP027423:c1043402-1042860 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T43943 NZ_AP027423:c1043402-1042860 [Escherichia coli]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTACTTATCCTGGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATCGGCATCTGTACTTTTACGGCATATTCGCTGGAAAAA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAAATTGGTGTTGTCAGATTAGTCATGTTGGGGGTAGCACG
GAAGTATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAATAATTCACCAGGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAATTTTTATGCTCGTCTCGGCTTTGTTCAGCTTTCAGCG
ACACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT43943 WP_001297137.1 NZ_AP027423:c1043697-1043386 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT43943 NZ_AP027423:c1043697-1043386 [Escherichia coli]
ATGCCTGGAAAAACAGCCACTCTACCCGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAACTCAAAACTAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Structures

Toxin

• PDB: 7BYY

Antitoxin

• AlphaFold DB: A0A0E0Y6B3