Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) chpSB/PRK09812-ChpS
Location 381774..382369 Replicon chromosome
Accession NZ_AP027423
Organism Escherichia coli strain EC261 isolate EC261

Toxin (Protein)


Gene name chpB Uniprot ID U9Y4M4
Locus tag QUE58_RS04310 Protein ID WP_000239579.1
Coordinates 382019..382369 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name chpS Uniprot ID U9Y2K1
Locus tag QUE58_RS04305 Protein ID WP_001223208.1
Coordinates 381774..382025 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QUE58_RS04295 (377439) 377439..381218 + 3780 WP_000060895.1 autotransporter assembly complex protein TamB -
QUE58_RS04300 (381221) 381221..381562 + 342 WP_001219160.1 gamma-glutamylcyclotransferase -
QUE58_RS04305 (381774) 381774..382025 + 252 WP_001223208.1 type II toxin-antitoxin system antitoxin ChpS Antitoxin
QUE58_RS04310 (382019) 382019..382369 + 351 WP_000239579.1 endoribonuclease toxin ChpB Toxin
QUE58_RS04315 (382449) 382449..382979 - 531 WP_000055072.1 inorganic diphosphatase -
QUE58_RS04320 (383289) 383289..384245 + 957 WP_000265933.1 galactofuranose ABC transporter substrate-binding protein YtfQ -
QUE58_RS04325 (384555) 384555..386057 + 1503 WP_000205794.1 sugar ABC transporter ATP-binding protein -
QUE58_RS04330 (386071) 386071..387093 + 1023 WP_001296689.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(8-114)

Antitoxin

SpoVT-AbrB domain

(6-49)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12511.45 Da        Isoelectric Point: 5.6219

>T43939 WP_000239579.1 NZ_AP027423:382019-382369 [Escherichia coli]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAADEVVEEALLRLQAVVE

Download         Length: 351 bp

>T43939 NZ_AP027423:382019-382369 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9271.58 Da        Isoelectric Point: 4.4201

>AT43939 WP_001223208.1 NZ_AP027423:381774-382025 [Escherichia coli]
MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEAQVSNNQLILTPISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGD
EIW

Download         Length: 252 bp

>AT43939 NZ_AP027423:381774-382025 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y8A8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LQ26

References