Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 278798..279295 Replicon chromosome
Accession NZ_AP027423
Organism Escherichia coli strain EC261 isolate EC261

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag QUE58_RS03785 Protein ID WP_001173343.1
Coordinates 279122..279295 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag QUE58_RS03780 Protein ID WP_000398619.1
Coordinates 278798..279094 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QUE58_RS03760 (275529) 275529..276248 - 720 WP_000611288.1 two-component system response regulator DcuR -
QUE58_RS03765 (276245) 276245..277876 - 1632 WP_001216487.1 sensor histidine kinase -
QUE58_RS03770 (278057) 278057..278287 + 231 WP_000371700.1 4Fe-4S mono-cluster protein YjdI -
QUE58_RS03775 (278299) 278299..278571 + 273 WP_000405649.1 GNAT family N-acetyltransferase -
QUE58_RS03780 (278798) 278798..279094 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
QUE58_RS03785 (279122) 279122..279295 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
QUE58_RS03790 (279414) 279414..280931 - 1518 WP_001295074.1 lysine--tRNA ligase -
QUE58_RS03795 (281168) 281168..282625 - 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T43938 WP_001173343.1 NZ_AP027423:279122-279295 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T43938 NZ_AP027423:279122-279295 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT43938 WP_000398619.1 NZ_AP027423:278798-279094 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT43938 NZ_AP027423:278798-279094 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References