Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4523603..4524100 Replicon chromosome
Accession NZ_AP027419
Organism Escherichia coli strain EC476 isolate EC476

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag QUE76_RS23120 Protein ID WP_001173343.1
Coordinates 4523603..4523776 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag QUE76_RS23125 Protein ID WP_000398619.1
Coordinates 4523804..4524100 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QUE76_RS23110 (4520273) 4520273..4521730 + 1458 WP_022646457.1 dipeptide/tripeptide permease DtpC -
QUE76_RS23115 (4521967) 4521967..4523484 + 1518 WP_000003800.1 lysine--tRNA ligase -
QUE76_RS23120 (4523603) 4523603..4523776 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
QUE76_RS23125 (4523804) 4523804..4524100 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
QUE76_RS23130 (4524329) 4524329..4524601 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
QUE76_RS23135 (4524613) 4524613..4524843 - 231 WP_001683478.1 4Fe-4S mono-cluster protein YjdI -
QUE76_RS23140 (4525024) 4525024..4526655 + 1632 WP_022646455.1 sensor histidine kinase -
QUE76_RS23145 (4526652) 4526652..4527371 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T43922 WP_001173343.1 NZ_AP027419:c4523776-4523603 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T43922 NZ_AP027419:c4523776-4523603 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTAGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT43922 WP_000398619.1 NZ_AP027419:c4524100-4523804 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT43922 NZ_AP027419:c4524100-4523804 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACCGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References