Detailed information of TA system TA043920

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-RelB
• Location: 4483486..4484084
• Replicon: chromosome
• Accession: NZ_AP027419
• Organism: Escherichia coli strain EC476 isolate EC476

Toxin (Protein)

• Gene name: doc
• Uniprot ID: A0A8T6PRV3
• Locus tag: QUE76_RS22925
• Protein ID: WP_053291281.1
• Coordinates: 4483486..4483863 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: L4J1C0
• Locus tag: QUE76_RS22930
• Protein ID: WP_001603498.1
• Coordinates: 4483863..4484084 (-)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QUE76_RS22895 (4478710)	4478710..4479048	+	339	WP_000883400.1	divalent cation tolerance protein CutA	-
QUE76_RS22900 (4479024)	4479024..4480721	+	1698	WP_000068927.1	protein-disulfide reductase DsbD	-
QUE76_RS22905 (4480758)	4480758..4481333	+	576	WP_001188520.1	transcriptional regulator	-
QUE76_RS22915 (4481713)	4481713..4482975	+	1263	WP_063078140.1	integrase arm-type DNA-binding domain-containing protein	-
QUE76_RS22920 (4483161)	4483161..4483376	+	216	Protein_4198	transposase	-
QUE76_RS22925 (4483486)	4483486..4483863	-	378	WP_053291281.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
QUE76_RS22930 (4483863)	4483863..4484084	-	222	WP_001603498.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
QUE76_RS22935 (4484269)	4484269..4485288	-	1020	WP_286347852.1	fimbrial protein	-
QUE76_RS22940 (4485300)	4485300..4487786	-	2487	WP_111970038.1	fimbrial biogenesis outer membrane usher protein	-
QUE76_RS22945 (4487804)	4487804..4488484	-	681	WP_286347853.1	fimbrial assembly chaperone	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	4480758..4505899	25141
	-	flank	IS/Tn	-	-	4483161..4483442	281

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Fido domain (4-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-51)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13487.28 Da
• Isoelectric Point: 6.2257

>T43920 WP_053291281.1 NZ_AP027419:c4483863-4483486 [Escherichia coli]
MRHISPEELIAIHDANISRYGGLPGMPDSGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVYDSPELAELTVGAATGEVSVSTVVATLRRLYGTA

Nucleotide

• Length: 378 bp

>T43920 NZ_AP027419:c4483863-4483486 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAGCTTATTGCAATTCATGATGCGAATATAAGCCGCTACGGCGGCCTGCCAGGCATGCC
AGATTCGGGAAGAGCTGAGGCTATTATCGGGAGAGTCCAGGCCAGAGTTGCATATGAAGAGATTACCGACCTTTTCGAAG
TCTCCGCAACTTACCTGGTGGCTACTGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
TTGTTATTTCTGCGCCGTAACGGTGTGCAGGTATATGATTCACCAGAACTGGCAGAGCTTACTGTGGGGGCTGCGACCGG
AGAGGTATCCGTATCTACTGTCGTTGCCACGTTACGTAGATTGTATGGTACGGCGTAG

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8190.24 Da
• Isoelectric Point: 6.3159

>AT43920 WP_001603498.1 NZ_AP027419:c4484084-4483863 [Escherichia coli]
MQSINFRTARSNLTEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDTTNKKLVNR

Nucleotide

• Length: 222 bp

>AT43920 NZ_AP027419:c4484084-4483863 [Escherichia coli]
ATGCAATCTATTAACTTCCGTACCGCACGCAGCAATCTTACCGAAGTGCTCAACAATGTTGAAGCCGGAGAAGAGGTTGA
AATCACCCGTAGAGGCCGTGAGCCAGCTGTAATTGTCAGCAAGGCCACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTCGCATCCCTGTTTGACACTCTGGACACCACCAATAAGAAACTGGTTAACCGATAA

Structures

Antitoxin

• AlphaFold DB: L4J1C0