Detailed information of TA system TA043919

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): chpSB/PRK09812-ChpS
• Location: 4389257..4389852
• Replicon: chromosome
• Accession: NZ_AP027419
• Organism: Escherichia coli strain EC476 isolate EC476

Toxin (Protein)

• Gene name: chpB
• Uniprot ID: V0SXT4
• Locus tag: QUE76_RS22425
• Protein ID: WP_000239581.1
• Coordinates: 4389257..4389607 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: chpS
• Uniprot ID: L4JJX7
• Locus tag: QUE76_RS22430
• Protein ID: WP_001223213.1
• Coordinates: 4389601..4389852 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QUE76_RS22405 (4384711)	4384711..4385733	-	1023	WP_001298067.1	ABC transporter permease	-
QUE76_RS22410 (4385747)	4385747..4387249	-	1503	WP_022646474.1	sugar ABC transporter ATP-binding protein	-
QUE76_RS22415 (4387382)	4387382..4388338	-	957	WP_000265933.1	galactofuranose ABC transporter substrate-binding protein YtfQ	-
QUE76_RS22420 (4388648)	4388648..4389178	+	531	WP_000055075.1	inorganic diphosphatase	-
QUE76_RS22425 (4389257)	4389257..4389607	-	351	WP_000239581.1	endoribonuclease toxin ChpB	Toxin
QUE76_RS22430 (4389601)	4389601..4389852	-	252	WP_001223213.1	type II toxin-antitoxin system antitoxin ChpS	Antitoxin
QUE76_RS22435 (4390064)	4390064..4390405	-	342	WP_001219160.1	gamma-glutamylcyclotransferase	-
QUE76_RS22440 (4390408)	4390408..4394187	-	3780	WP_022646473.1	autotransporter assembly complex protein TamB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (8-114)

Antitoxin

• SpoVT-AbrB domain (6-49)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12453.41 Da
• Isoelectric Point: 6.2206

>T43919 WP_000239581.1 NZ_AP027419:c4389607-4389257 [Escherichia coli]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAAGEVVEEALLRLQAVVE

Nucleotide

• Length: 351 bp

>T43919 NZ_AP027419:c4389607-4389257 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGGTGAGGTGGTGGAAGAGGC
GTTATTACGTTTGCAGGCGGTGGTGGAATAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9331.64 Da
• Isoelectric Point: 4.6509

>AT43919 WP_001223213.1 NZ_AP027419:c4389852-4389601 [Escherichia coli]
MRITIKRWGNSSGMVIPNVVMKELNLRPGQSVEAQVSNNQLILTPISRRYSLDELLAQCDMNATELSEQDVWGKSTPAGD
EIW

Nucleotide

• Length: 252 bp

>AT43919 NZ_AP027419:c4389852-4389601 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTTCAGGTATGGTCATTCCTAATGTTGTGATGAAAGAACTTAACTTACG
GCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCACGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Structures

Toxin

• AlphaFold DB: V0SXT4

Antitoxin

• AlphaFold DB: L4JJX7