Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 4063389..4063939 Replicon chromosome
Accession NZ_AP027419
Organism Escherichia coli strain EC476 isolate EC476

Toxin (Protein)


Gene name ccdB Uniprot ID S1PDG4
Locus tag QUE76_RS20975 Protein ID WP_001160966.1
Coordinates 4063389..4063703 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1NMQ0
Locus tag QUE76_RS20980 Protein ID WP_000125568.1
Coordinates 4063706..4063939 (-) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QUE76_RS20950 (4059064) 4059064..4060350 + 1287 WP_000800457.1 peptidylprolyl isomerase SurA -
QUE76_RS20955 (4060350) 4060350..4061339 + 990 WP_000241224.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
QUE76_RS20960 (4061336) 4061336..4062157 + 822 WP_001065381.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
QUE76_RS20965 (4062160) 4062160..4062537 + 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
QUE76_RS20970 (4062544) 4062544..4063392 + 849 WP_000257195.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
QUE76_RS20975 (4063389) 4063389..4063703 - 315 WP_001160966.1 CcdB family protein Toxin
QUE76_RS20980 (4063706) 4063706..4063939 - 234 WP_000125568.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
QUE76_RS20985 (4064025) 4064025..4064504 - 480 WP_000624375.1 type 3 dihydrofolate reductase -
QUE76_RS20990 (4064696) 4064696..4066558 - 1863 WP_022645165.1 glutathione-regulated potassium-efflux system protein KefC -
QUE76_RS20995 (4066551) 4066551..4067081 - 531 WP_000600732.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
QUE76_RS21000 (4067189) 4067189..4068520 - 1332 WP_001183201.1 MFS transporter -
QUE76_RS21005 (4068578) 4068578..4068865 - 288 WP_000203741.1 ferredoxin-like protein FixX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-103)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11711.72 Da        Isoelectric Point: 9.0851

>T43918 WP_001160966.1 NZ_AP027419:c4063703-4063389 [Escherichia coli]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Download         Length: 315 bp

>T43918 NZ_AP027419:c4063703-4063389 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCGTTTCCCTTTGTTATCGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCCGTATCGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAATC
CAATCCTTTTACTGGTAGATGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCACACCGAACGTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8560.55 Da        Isoelectric Point: 5.8062

>AT43918 WP_000125568.1 NZ_AP027419:c4063939-4063706 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENTEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT43918 NZ_AP027419:c4063939-4063706 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACA
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G815

Antitoxin

Source ID Structure
AlphaFold DB A0A829GEF2

References