Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3582238..3582856 Replicon chromosome
Accession NZ_AP027419
Organism Escherichia coli strain EC476 isolate EC476

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag QUE76_RS18645 Protein ID WP_001291435.1
Coordinates 3582638..3582856 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag QUE76_RS18640 Protein ID WP_000344800.1
Coordinates 3582238..3582612 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QUE76_RS18630 (3577327) 3577327..3578520 + 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
QUE76_RS18635 (3578543) 3578543..3581692 + 3150 WP_112918615.1 efflux RND transporter permease AcrB -
QUE76_RS18640 (3582238) 3582238..3582612 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
QUE76_RS18645 (3582638) 3582638..3582856 + 219 WP_001291435.1 HHA domain-containing protein Toxin
QUE76_RS18650 (3583028) 3583028..3583579 + 552 WP_000536391.1 maltose O-acetyltransferase -
QUE76_RS18655 (3583695) 3583695..3584165 + 471 WP_022645280.1 YlaC family protein -
QUE76_RS18660 (3584329) 3584329..3585879 + 1551 WP_012602175.1 cyclic-guanylate-specific phosphodiesterase PdeB -
QUE76_RS18665 (3585920) 3585920..3586273 - 354 WP_000878140.1 DUF1428 domain-containing protein -
QUE76_RS18675 (3586652) 3586652..3586963 + 312 WP_000409911.1 MGMT family protein -
QUE76_RS18680 (3586994) 3586994..3587566 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T43916 WP_001291435.1 NZ_AP027419:3582638-3582856 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T43916 NZ_AP027419:3582638-3582856 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT43916 WP_000344800.1 NZ_AP027419:3582238-3582612 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT43916 NZ_AP027419:3582238-3582612 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References