Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 2574783..2575421 Replicon chromosome
Accession NZ_AP027419
Organism Escherichia coli strain EC476 isolate EC476

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag QUE76_RS13940 Protein ID WP_000813794.1
Coordinates 2575245..2575421 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag QUE76_RS13935 Protein ID WP_001270286.1
Coordinates 2574783..2575199 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QUE76_RS13915 (2569935) 2569935..2570876 - 942 WP_088129997.1 ABC transporter permease -
QUE76_RS13920 (2570877) 2570877..2571890 - 1014 WP_022645666.1 ABC transporter ATP-binding protein -
QUE76_RS13925 (2571908) 2571908..2573053 - 1146 WP_000047466.1 ABC transporter substrate-binding protein -
QUE76_RS13930 (2573298) 2573298..2574704 - 1407 WP_022645665.1 PLP-dependent aminotransferase family protein -
QUE76_RS13935 (2574783) 2574783..2575199 - 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
QUE76_RS13940 (2575245) 2575245..2575421 - 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
QUE76_RS13945 (2575643) 2575643..2575873 + 231 WP_000491567.1 DUF2554 family protein -
QUE76_RS13950 (2575965) 2575965..2577926 - 1962 WP_023909195.1 23S rRNA 5-hydroxycytidine C2501 synthase -
QUE76_RS13955 (2577999) 2577999..2578535 - 537 WP_000429148.1 DNA-binding transcriptional regulator SutR -
QUE76_RS13960 (2578627) 2578627..2579799 + 1173 WP_022645663.1 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T43912 WP_000813794.1 NZ_AP027419:c2575421-2575245 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T43912 NZ_AP027419:c2575421-2575245 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTAAAACTGAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT43912 WP_001270286.1 NZ_AP027419:c2575199-2574783 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT43912 NZ_AP027419:c2575199-2574783 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACAGTTGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCGTTACCTTCGCCATTAAACAGTCACGATCACTTTATTGAAGTGCCTTTGAGCGTCGCCTCTAAAGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAATTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCTAAGGCGCTTGGCAAAGAGTTAT
CACTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References