Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4375427..4376045 Replicon chromosome
Accession NZ_AP027417
Organism Escherichia coli strain EC361 isolate EC361

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag QUF17_RS24580 Protein ID WP_001291435.1
Coordinates 4375427..4375645 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag QUF17_RS24585 Protein ID WP_000344800.1
Coordinates 4375671..4376045 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QUF17_RS24545 (4370717) 4370717..4371289 + 573 WP_000779826.1 YbaY family lipoprotein -
QUF17_RS24550 (4371320) 4371320..4371631 - 312 WP_000409911.1 MGMT family protein -
QUF17_RS24560 (4372010) 4372010..4372363 + 354 WP_000878141.1 DUF1428 family protein -
QUF17_RS24565 (4372405) 4372405..4373955 - 1551 WP_001299455.1 cyclic-guanylate-specific phosphodiesterase PdeB -
QUF17_RS24570 (4374119) 4374119..4374589 - 471 WP_000136192.1 YlaC family protein -
QUF17_RS24575 (4374705) 4374705..4375256 - 552 WP_000102564.1 maltose O-acetyltransferase -
QUF17_RS24580 (4375427) 4375427..4375645 - 219 WP_001291435.1 HHA domain-containing protein Toxin
QUF17_RS24585 (4375671) 4375671..4376045 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
QUF17_RS24590 (4376591) 4376591..4379740 - 3150 WP_001132469.1 efflux RND transporter permease AcrB -
QUF17_RS24595 (4379763) 4379763..4380956 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T43897 WP_001291435.1 NZ_AP027417:c4375645-4375427 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T43897 NZ_AP027417:c4375645-4375427 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTATACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT43897 WP_000344800.1 NZ_AP027417:c4376045-4375671 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT43897 NZ_AP027417:c4376045-4375671 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References