Detailed information of TA system TA043896

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 4341132..4341969
• Replicon: chromosome
• Accession: NZ_AP027417
• Organism: Escherichia coli strain EC361 isolate EC361

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: Q3Z4X7
• Locus tag: QUF17_RS24410
• Protein ID: WP_000227784.1
• Coordinates: 4341132..4341674 (-)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: QUF17_RS24415
• Protein ID: WP_001297137.1
• Coordinates: 4341658..4341969 (-)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QUF17_RS24390 (4336671)	4336671..4337582	-	912	WP_000705874.1	2-dehydropantoate 2-reductase	-
QUF17_RS24395 (4337750)	4337750..4338241	+	492	WP_001138904.1	nucleotide binding protein YajQ	-
QUF17_RS24400 (4338369)	4338369..4339733	-	1365	WP_001000974.1	MFS transporter	-
QUF17_RS24405 (4340141)	4340141..4341076	+	936	WP_001365761.1	tetratricopeptide repeat protein	-
QUF17_RS24410 (4341132)	4341132..4341674	-	543	WP_000227784.1	GNAT family N-acetyltransferase	Toxin
QUF17_RS24415 (4341658)	4341658..4341969	-	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
QUF17_RS24420 (4342154)	4342154..4343044	-	891	WP_000971336.1	heme o synthase	-
QUF17_RS24425 (4343056)	4343056..4343385	-	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
QUF17_RS24430 (4343385)	4343385..4343999	-	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
QUF17_RS24435 (4343989)	4343989..4345980	-	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
QUF17_RS24440 (4346002)	4346002..4346949	-	948	WP_001239441.1	cytochrome o ubiquinol oxidase subunit II	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19805.02 Da
• Isoelectric Point: 8.3395

>T43896 WP_000227784.1 NZ_AP027417:c4341674-4341132 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T43896 NZ_AP027417:c4341674-4341132 [Escherichia coli]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTACTTATCCTGGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATCGGCATCTGTACTTTTACGGCATATTCGCTGGAAAAA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAAATTGGTGTTGTCAGATTAGTCATGTTGGGGGTAGCACG
GAAGTATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAATAATTCACCAGGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAATTTTTATGCTCGTCTCGGCTTTGTTCAGCTTTCAGCG
ACACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT43896 WP_001297137.1 NZ_AP027417:c4341969-4341658 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT43896 NZ_AP027417:c4341969-4341658 [Escherichia coli]
ATGCCTGGAAAAACAGCCACTCTACCCGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAACTCAAAACTAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Structures

Toxin

• PDB: 7BYY

Antitoxin

• AlphaFold DB: A0A0E0Y6B3