Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 2746162..2746793 Replicon chromosome
Accession NZ_AP027417
Organism Escherichia coli strain EC361 isolate EC361

Toxin (Protein)


Gene name hicA Uniprot ID F4NNK6
Locus tag QUF17_RS16900 Protein ID WP_001259388.1
Coordinates 2746518..2746793 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag QUF17_RS16895 Protein ID WP_000593555.1
Coordinates 2746162..2746521 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QUF17_RS16870 (2742294) 2742294..2743238 + 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -
QUF17_RS16875 (2743235) 2743235..2744068 + 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
QUF17_RS16880 (2744068) 2744068..2744832 + 765 WP_001136236.1 nickel import ATP-binding protein NikD -
QUF17_RS16885 (2744829) 2744829..2745635 + 807 WP_000173666.1 nickel import ATP-binding protein NikE -
QUF17_RS16890 (2745641) 2745641..2746042 + 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
QUF17_RS16895 (2746162) 2746162..2746521 - 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
QUF17_RS16900 (2746518) 2746518..2746793 - 276 WP_001259388.1 type II toxin-antitoxin system HicA family toxin Toxin
QUF17_RS16905 (2746866) 2746866..2747990 - 1125 WP_001314210.1 ABC transporter permease -
QUF17_RS16910 (2747990) 2747990..2750725 - 2736 WP_000149125.1 ribosome-associated ATPase/putative transporter RbbA -
QUF17_RS16915 (2750722) 2750722..2751789 - 1068 WP_000361485.1 HlyD family secretion protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10151.89 Da        Isoelectric Point: 10.7076

>T43891 WP_001259388.1 NZ_AP027417:c2746793-2746518 [Escherichia coli]
MRTKVQALRKKQKNTLDQIVKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T43891 NZ_AP027417:c2746793-2746518 [Escherichia coli]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATAGTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGCAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT43891 WP_000593555.1 NZ_AP027417:c2746521-2746162 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT43891 NZ_AP027417:c2746521-2746162 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCAGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A0FFA2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References