Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 2109474..2110128 Replicon chromosome
Accession NZ_AP027417
Organism Escherichia coli strain EC361 isolate EC361

Toxin (Protein)


Gene name cptA Uniprot ID F4NJ21
Locus tag QUF17_RS13685 Protein ID WP_000244772.1
Coordinates 2109474..2109881 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag QUF17_RS13690 Protein ID WP_000354046.1
Coordinates 2109862..2110128 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QUF17_RS13665 (2105431) 2105431..2107164 - 1734 WP_000813215.1 single-stranded-DNA-specific exonuclease RecJ -
QUF17_RS13670 (2107170) 2107170..2107880 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
QUF17_RS13675 (2107905) 2107905..2108801 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
QUF17_RS13680 (2108913) 2108913..2109434 + 522 WP_001055874.1 flavodoxin FldB -
QUF17_RS13685 (2109474) 2109474..2109881 - 408 WP_000244772.1 protein YgfX Toxin
QUF17_RS13690 (2109862) 2109862..2110128 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
QUF17_RS13695 (2110371) 2110371..2111351 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
QUF17_RS13700 (2111428) 2111428..2112087 - 660 WP_000250274.1 hemolysin III family protein -
QUF17_RS13705 (2112251) 2112251..2112562 - 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
QUF17_RS13710 (2112607) 2112607..2114040 + 1434 WP_001344773.1 6-phospho-beta-glucosidase BglA -
QUF17_RS13715 (2114097) 2114097..2114840 - 744 WP_000951964.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16048.02 Da        Isoelectric Point: 11.2511

>T43888 WP_000244772.1 NZ_AP027417:c2109881-2109474 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T43888 NZ_AP027417:c2109881-2109474 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGTGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT43888 WP_000354046.1 NZ_AP027417:c2110128-2109862 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT43888 NZ_AP027417:c2110128-2109862 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYB4

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References