Detailed information of TA system TA043886

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 1566294..1566919
• Replicon: chromosome
• Accession: NZ_AP027417
• Organism: Escherichia coli strain EC361 isolate EC361

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: QUF17_RS10935
• Protein ID: WP_000911330.1
• Coordinates: 1566294..1566692 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: QUF17_RS10940
• Protein ID: WP_000450524.1
• Coordinates: 1566692..1566919 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QUF17_RS10915 (1562201)	1562201..1562401	+	201	WP_000383836.1	YpfN family protein	-
QUF17_RS10920 (1562482)	1562482..1563180	-	699	WP_000679812.1	esterase	-
QUF17_RS10925 (1563254)	1563254..1565269	-	2016	WP_000829292.1	tRNA cytosine(34) acetyltransferase TmcA	-
QUF17_RS10930 (1565284)	1565284..1566147	-	864	WP_001267498.1	neutral zinc metallopeptidase	-
QUF17_RS10935 (1566294)	1566294..1566692	-	399	WP_000911330.1	type II toxin-antitoxin system VapC family toxin	Toxin
QUF17_RS10940 (1566692)	1566692..1566919	-	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
QUF17_RS10945 (1567073)	1567073..1567786	-	714	WP_001347624.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
QUF17_RS10950 (1567999)	1567999..1569033	-	1035	WP_001330579.1	outer membrane protein assembly factor BamC	-
QUF17_RS10955 (1569050)	1569050..1569928	-	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
QUF17_RS10960 (1570074)	1570074..1570646	+	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
QUF17_RS10965 (1570646)	1570646..1571116	+	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T43886 WP_000911330.1 NZ_AP027417:c1566692-1566294 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T43886 NZ_AP027417:c1566692-1566294 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT43886 WP_000450524.1 NZ_AP027417:c1566919-1566692 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT43886 NZ_AP027417:c1566919-1566692 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33