Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 438549..439187 Replicon chromosome
Accession NZ_AP027417
Organism Escherichia coli strain EC361 isolate EC361

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag QUF17_RS05440 Protein ID WP_000813794.1
Coordinates 438549..438725 (+) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag QUF17_RS05445 Protein ID WP_001270286.1
Coordinates 438771..439187 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QUF17_RS05420 (434168) 434168..435343 - 1176 WP_001236215.1 BenE family transporter YdcO -
QUF17_RS05425 (435435) 435435..435971 + 537 WP_000429155.1 DNA-binding transcriptional regulator SutR -
QUF17_RS05430 (436044) 436044..438005 + 1962 WP_001593323.1 23S rRNA 5-hydroxycytidine C2501 synthase -
QUF17_RS05435 (438097) 438097..438327 - 231 WP_000494244.1 YncJ family protein -
QUF17_RS05440 (438549) 438549..438725 + 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
QUF17_RS05445 (438771) 438771..439187 + 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
QUF17_RS05450 (439266) 439266..440672 + 1407 WP_286338189.1 PLP-dependent aminotransferase family protein -
QUF17_RS05455 (440917) 440917..442062 + 1146 WP_000047456.1 ABC transporter substrate-binding protein -
QUF17_RS05460 (442080) 442080..443093 + 1014 WP_000220396.1 ABC transporter ATP-binding protein -
QUF17_RS05465 (443094) 443094..444035 + 942 WP_001251315.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 432980..434128 1148


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T43874 WP_000813794.1 NZ_AP027417:438549-438725 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T43874 NZ_AP027417:438549-438725 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT43874 WP_001270286.1 NZ_AP027417:438771-439187 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT43874 NZ_AP027417:438771-439187 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References