Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-DnaT
Location 84157..84679 Replicon plasmid pEC361_2
Accession NZ_AP027415
Organism Escherichia coli strain EC361 isolate EC361

Toxin (Protein)


Gene name relE Uniprot ID G3CAN5
Locus tag QUF17_RS01765 Protein ID WP_000220560.1
Coordinates 84398..84679 (+) Length 94 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID G3CAG1
Locus tag QUF17_RS01760 Protein ID WP_000121743.1
Coordinates 84157..84408 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QUF17_RS01740 (79625) 79625..80329 + 705 WP_001067855.1 IS6-like element IS26 family transposase -
QUF17_RS01745 (80478) 80478..81665 + 1188 WP_000937591.1 IS91 family transposase -
QUF17_RS01750 (81854) 81854..82051 - 198 Protein_91 hypothetical protein -
QUF17_RS01755 (82091) 82091..82927 - 837 WP_001050931.1 RepB family plasmid replication initiator protein -
QUF17_RS01760 (84157) 84157..84408 + 252 WP_000121743.1 plasmid stabilization protein Antitoxin
QUF17_RS01765 (84398) 84398..84679 + 282 WP_000220560.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
QUF17_RS01770 (85062) 85062..86249 - 1188 WP_000937614.1 IS91-like element IS91 family transposase -
QUF17_RS01775 (86249) 86249..86614 - 366 WP_000124098.1 hypothetical protein -
QUF17_RS01780 (86702) 86702..89338 - 2637 Protein_97 Tn3-like element TnAs3 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid sul3 / ant(3'')-Ia / dfrA12 / aadA2 hlyC / hlyA / hlyB / hlyD 1..104272 104272
- inside IScluster/Tn sul3 / ant(3'')-Ia / dfrA12 / aadA2 - 71001..102072 31071


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 11005.83 Da        Isoelectric Point: 10.5938

>T43866 WP_000220560.1 NZ_AP027415:84398-84679 [Escherichia coli]
MTYELAFDRRALKEWQKLGHTIREQFKKKLAERLENPRVPAARLHGHADRYKIKLRASGYRLVYQVIDEKVVLLVIAVGR
RESSEVYQIADLR

Download         Length: 282 bp

>T43866 NZ_AP027415:84398-84679 [Escherichia coli]
ATGACCTATGAACTGGCTTTTGACCGTAGAGCACTGAAGGAATGGCAGAAACTCGGCCACACCATCCGTGAACAATTCAA
AAAGAAACTGGCAGAACGGCTGGAAAATCCACGCGTACCCGCAGCCCGGTTACATGGTCATGCTGATCGCTATAAGATCA
AACTTCGTGCATCTGGCTACAGACTTGTATACCAAGTCATTGATGAGAAAGTCGTTTTACTCGTTATTGCCGTTGGAAGA
AGGGAAAGCAGCGAAGTCTATCAGATCGCAGATTTGCGCTAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9194.43 Da        Isoelectric Point: 4.1683

>AT43866 WP_000121743.1 NZ_AP027415:84157-84408 [Escherichia coli]
MSYQILTTTAASITDLKKNPMGTVAEGEGDAVAILNRNEPAFYCVPPKLYAYYRELAEDAELNAVADERMKNPEIVKVNL
DDL

Download         Length: 252 bp

>AT43866 NZ_AP027415:84157-84408 [Escherichia coli]
ATGAGCTATCAAATTCTGACAACCACAGCGGCCAGTATTACTGACCTGAAAAAAAATCCTATGGGAACCGTAGCTGAAGG
TGAAGGGGACGCTGTTGCGATCCTGAACCGAAACGAACCGGCGTTCTATTGCGTTCCACCAAAACTTTACGCCTACTATC
GGGAACTCGCTGAAGATGCTGAGTTAAACGCTGTTGCTGATGAGCGCATGAAAAACCCGGAAATTGTGAAGGTTAACCTG
GATGACCTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB G3CAN5

Antitoxin

Source ID Structure
AlphaFold DB A0A3S5I301

References