Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) parDE/RelE-RHH
Location 7251..7798 Replicon plasmid pEC361_2
Accession NZ_AP027415
Organism Escherichia coli strain EC361 isolate EC361

Toxin (Protein)


Gene name parE Uniprot ID G7QEJ9
Locus tag QUF17_RS01340 Protein ID WP_001384452.1
Coordinates 7520..7798 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name parD Uniprot ID E1QMW3
Locus tag QUF17_RS01335 Protein ID WP_000079941.1
Coordinates 7251..7520 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QUF17_RS01310 (3165) 3165..3530 - 366 WP_000124098.1 hypothetical protein -
QUF17_RS01320 (4563) 4563..4949 + 387 Protein_5 transposase -
QUF17_RS01325 (5015) 5015..5812 - 798 WP_032235557.1 IS21-like element IS21 family helper ATPase IstB -
QUF17_RS01330 (5812) 5812..6984 - 1173 WP_015056461.1 IS21-like element IS21 family transposase -
QUF17_RS01335 (7251) 7251..7520 + 270 WP_000079941.1 type II toxin-antitoxin system antitoxin YacA Antitoxin
QUF17_RS01340 (7520) 7520..7798 + 279 WP_001384452.1 type II toxin-antitoxin system toxin YacB Toxin
QUF17_RS01345 (8107) 8107..9249 + 1143 WP_158649904.1 YiiX/YebB-like N1pC/P60 family cysteine hydrolase -
QUF17_RS01350 (9222) 9222..9326 + 105 Protein_11 hypothetical protein -
QUF17_RS01355 (9338) 9338..9643 + 306 WP_000482654.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid sul3 / ant(3'')-Ia / dfrA12 / aadA2 hlyC / hlyA / hlyB / hlyD 1..104272 104272
- inside IScluster/Tn - - 1978..18319 16341


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(3-91)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10762.38 Da        Isoelectric Point: 8.4615

>T43865 WP_001384452.1 NZ_AP027415:7520-7798 [Escherichia coli]
MEIFWTMLASQDRKRIREYIAEQNLMAAIELDERIGYSASSLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKVY
ILRVLHTAQKWP

Download         Length: 279 bp

>T43865 NZ_AP027415:7520-7798 [Escherichia coli]
ATGGAGATATTCTGGACCATGCTGGCAAGCCAGGACAGAAAGCGCATTCGTGAGTACATTGCGGAGCAGAACCTGATGGC
CGCCATTGAACTGGACGAACGGATTGGTTATTCAGCCAGCAGCCTGGCCGGGCAGCCGTATAAAGGTCGCAATGGTCGTG
TGGAAGGTACCCGGGAACTGGTTATTCACCCGCATTTTGTTCTGGTTTATGAGGTCGATAGCCAGTGGGGAAAAGTGTAT
ATTCTGCGAGTGTTGCATACAGCACAGAAGTGGCCATAA

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10252.58 Da        Isoelectric Point: 7.2057

>AT43865 WP_000079941.1 NZ_AP027415:7251-7520 [Escherichia coli]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVEAGRQQLERGDVLPHDMVESSAAAWRD
EMSRKVAGK

Download         Length: 270 bp

>AT43865 NZ_AP027415:7251-7520 [Escherichia coli]
ATGGCACAGGTTAATATGAGTTTAAGAATCGACGCTGAACTGAAGGATGCTTTTATGGCCGCGGCAAAAAGCATGGACCG
TAATGGCTCTCAGTTAATCCGGGATTTTATGCGCCAGACCGTTGAACGGCAGCATAATACCTGGTTCCGTGACCAGGTTG
AGGCAGGACGTCAGCAACTCGAACGCGGCGATGTGCTTCCTCATGACATGGTCGAATCCTCCGCTGCTGCATGGCGGGAT
GAAATGAGCAGGAAGGTCGCCGGTAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CL36

Antitoxin

Source ID Structure
AlphaFold DB A0A629TXC5

References